Effect of proline-spirooxindole on viability and apoptosis of non-small-cell lung cancer A549 cells
10.3969/j.issn.1000-4718.2019.02.016
- VernacularTitle:吡咯烷螺二吲哚对非小细胞肺癌A549细胞活力及凋亡的影响
- Author:
Jin-Zhu MA
1
;
Liang YAN
;
Jia-Wei ZAN
;
Lei XU
;
Yi WANG
;
Gen-Bao ZHANG
Author Information
1. 安徽省多糖药物工程技术研究中心
- Keywords:
Non-small-cell lung cancer;
Proline-spirooxindole;
Cell viability;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2019;35(2):286-290
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of proline-spirooxindole on the viability and apoptosis of human nonsmall-cell lung cancer A549 cells.METHODS:The effect of proline-spirooxindole on the viability of A549 cells was determined by CCK-8 assay.The apoptosis was analyzed by flow cytometry.The effects of proline-spirooxindole on the expression of PARP and p53 and the phosphorylation of m TOR were determined by Western blot.RESULTS:After A549 cells were treated with proline-spirooxindole (25, 50 and 100 mg/L) , the cell viability was decreased (P<0.01) compared with DMSO control group.The apoptotic rate was increased compared with DMSO control group (P<0.01).The protein expression of p53 was up-regulated, the increased apoptotic protein cleaved PARP was observed, and the phosphorylation of m TOR was inhibited (P<0.01).CONCLUSION:Proline-spirooxindole inhibits the viability of A549 cells and induces apoptosis, which may be related to the phosphorylation of m TOR.
