Effect of urantide on liver function and histomorphology in atherosclerotic rats
10.3969/j.issn.1000-4718.2019.02.005
- VernacularTitle:Urantide对动脉粥样硬化大鼠肝功能及组织形态学的影响
- Author:
Hai-Peng CUI
1
;
Kai LIU
;
Tian-Jiao GUO
;
Xiao-Xu SUN
;
Tu WANG
;
Ya-Qin XIE
;
Ying LI
;
Guang-Xin MIAO
;
Juan ZHAO
Author Information
1. 承德医学院病理生理学教研室
- Keywords:
Urantide;
Atherosclerosis;
Liver function;
Urotensin Ⅱ;
Fatty degeneration
- From:
Chinese Journal of Pathophysiology
2019;35(2):218-223
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of urantide on the liver function and histomorphology in the rats with atherosclerosis (AS).METHODS:The AS Wistar rat model was induced by intraperitoneal injection of vitamin D3 (VD3) and feeding with high-fat diet.The rats were randomly divided into normal control group, AS model group, positive medicine group and urantide group.The liver function indexes of the rats were measured by biochemical test, and the pathological changes of the aorta and liver of the rats were observed by hematoxylin-eosin (HE) staining.The mRNA expression of urotensinⅡ (UII) and GPR14 at mRNA and protein levels in rat livers was determined by RT-qPCR and Western blot.RESULTS:The levels of alanine aminotransferase (ALT) , aspartate aminotransferase (AST) , γ-glutamyltransferase (γ-GT) , lactate dehydrogenase (LDH) , total bilirubin (TBIL) , indirect bilirubin (IBIL) and alkaline phosphatase (ALP) in AS model group were significantly increased compared with normal control group (P<0.05).The above indexes in urantide group were remarkably decreased compared with AS model group (P<0.05).No change of the levels of direct bilirubin (DBIL) , total protein (TP) , globulin (GLB) and albumin (ALB) in each group was observed.Urantide postponed hepatocyte fatty degeneration and repaired hepatocyte injury in the AS rats.Compared with normal control group, the mRNA and protein levels of UII and GPR14 in the liver were significantly increased in AS model group (P<0.05).With the prolongation of dosing time, the mRNA and protein levels of UII and GPR14 in the liver were significantly decreased in urantide group compared with AS model group (P<0.05).CONCLUSION:Urantide significantly attenuates the liver damage caused by liver fatty degeneration in AS rats.
