Effect of miRNA-33 on ABCA1/ABCG1 expression in RAW264.7 macrophage-derived foam cells after Hcy treatment
10.3969/j.issn.1000-4718.2019.02.004
- VernacularTitle:miRNA-33对Hcy干预RAW264.7巨噬细胞衍生的泡沫细胞表达ABCA1/ABCG1的影响
- Author:
Pei DAI
1
;
Fen GAO
;
Hong-Wei GAO
;
Yuan WANG
;
Gao-Jie FENG
;
Qin-Feng ZHANG
;
Rui BAI
;
Wei-Wei QIN
;
Hong LI
;
Xiao-Su SONG
Author Information
1. 山西医科大学第二医院心内科
- Keywords:
MicroRNA-33;
Homocysteine;
Cholesterol reverse transport;
ATP binding cassette transporter A1;
ATP binding cassette transporter G1
- From:
Chinese Journal of Pathophysiology
2019;35(2):212-217
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study whether homocysteine (Hcy) inhibits the expression of ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) by microRNA-33 (miRNA-33) signaling, and reduces the efficiency of reverse cholesterol transport (RCT).METHODS:RAW264.7 macrophages were induced by oxidized low-density lipoprotein (ox-LDL) to establish foam cell model.Oil red O staining was used to determine whether the model was established successfully.miRNA-33 mimics and miRNA-33 inhibitor were transfected into the cells by Lipofectamine 2000, and the cells were exposed to Hcy at concentration of 5 mmol/L for 24 h.The intracellular lipid droplets were observed by Oil red O staining.The expression of ABCA1 and ABCG1 at mRNA and protein levels was determined by real-time PCR and Western blot.The cellular cholesterol content was analyzed by HPLC, and effluent rate of cholesterol was detected by the method of liquid scintillation counting.RESULTS:Compared with blank control group, the lipid content in miRNA-33 mimics group was increased, and the expression of ABCA1 and ABCG1 at mRNA and protein levels was decreased (P<0.05).The intracellular cholesterol content was increased gradually (P<0.05) , and the cellular cholesterol efflux rate was gradually decreased (P<0.05) in miRNA-33 mimics group.Compared with blank control group, the testing results in miRNA-33 inhibitor group were the opposition of those in miRNA-33 mimics group (P<0.05).No difference of the above indexes among blank control group, miRNA-33 mimics-NC group and miRNA-33 inhibitor-NC group was observed.CONCLUSION:Hcy inhibits the mRNA and protein expression of ABCA1 and ABCG1 through miRNA-33 signaling, and reduces the efficiency of RCT in RAW264.7 macrophage-derived foam cells.
