Effect of 27nt-miRNA on regulation of SM22α expression in vascular smooth muscle cells and its effect on cell viability, migration and phenotypic changes
10.3969/j.issn.1000-4718.2019.02.002
- VernacularTitle:27nt-miRNA对血管平滑肌细胞SM22α表达的调节及其对细胞活力、迁移和表型改变的影响
- Author:
Feng SHEN
1
;
Peng YANG
;
Xiao-Jing TAO
;
Dan LI
;
Yuan-Yuan YAN
;
Xue-Lan LUO
;
Zu-Jie QIN
;
Yu-Wang QIN
;
He-Sheng OU
Author Information
1. 广西医科大学药学院
- Keywords:
27nt-miRNA;
Vascular smooth muscle cells;
Phenotype;
Smooth muscle 22α protein;
Platelet-derived growth factor
- From:
Chinese Journal of Pathophysiology
2019;35(2):200-205
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of 27nt-microRNA (27nt-miRNA) on the expression of smooth muscle 22α protein (SM22α) and the cell viability, migration and phenotypic changes of vascular smooth muscle cells (VSMCs).METHODS:The highly expression plasmids of 27nt-miRNA, and anti-27nt-miRNA and negative control plasmids were constructed, packaged with lentivirus and transfected into the rat primary VSMCs.Platelet-derived growth factor BB (PDGF-BB) was added to induce VSMCs phenotype conversion.The cell viability was measured by MTT assay.The migration ability was detected by scratch assay.The mRNA and protein expression of SM22αwas determined by RT-PCR, immunocytochemical staining and Western blot.RESULTS:Compared with normal group, the cell viability in PDGF-BB group was increased (P<0.05) , the migration ability was increased (P<0.05) and the expression of SM22αat mRNA and protein level was decreased (P<0.05).Compared with negative control lentiviral group, the cell viability in 27ntmiRNA over-expression group was decreased (P<0.05) , the migration ability was decreased (P<0.05) , and the mRNA and protein expression of SM22αwas increased (P<0.05).While in anti-27nt-miRNA group, the cell viability was increased (P<0.05) , the migration ability was increased (P<0.05) , and the mRNA and protein expression of SM22αwas decreased (P<0.05).CONCLUSION:27nt-miRNA significantly increases the expression of SM22α, while inhibits the viability and migration ability of VSMCs, and inhibits its phenotypic shift from contractile to synthetic.
