Pharmacokinetics of Three Kinds of Mangiferin Polymorphs in Rats
10.3870/j.issn.1004-0781.2019.02.014
- VernacularTitle:芒果苷3种晶型在大鼠体内药动学
- Author:
Haiguang YANG
1
;
Ying ZHAO
;
Shiying YANG
;
Lianhua FANG
;
Junke SONG
;
Yang LYU
;
Guanhua DU
Author Information
1. 中国医学科学院北京协和医学院药物研究所药物靶点研究与新药筛选北京市重点实验室
- Keywords:
Mangiferin;
Drug polymorphs;
HPLC-MS;
Plasma concentration;
Pharmacokinetics收稿日期2018-09-03修回日期2018-10-15
- From:
Herald of Medicine
2019;38(2):208-212
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mangiferin absorption process of mangiferin polymorphs in SD rats thus to find out the optimal crystal form and explore the factors that may affect the clinical effects of mangiferin. Methods Each rat was given one of three crystal forms of mangiferin. Plasma concentration of mangiferin were determined by HPLC-MS method. After liquidliquid extraction by ethyl acetate, the chromatographic separation was carried out on an Agilent ZORBAX SB-C18 (2.1 mm× 100 mm,3.5 μm) with a mobile phase consisting of methanol-0.1％ formic acid aqueous solution (30:70) . Mass spectrometry were performed in positive ion mode. Ion mass-to-charge ratio was set at 445 and 447 for mangiferin and, cefuroxime sodium (internal standard) respectivel for quantitive analysis. Results The main pharmacokinetic parameters of mangiferin form II, Ⅴ, Ⅵ were as follows: AUC(0-24 h) were (1323. 27 ± 218. 07) ,(1974. 34 ± 469. 24) ,(1737. 79 ± 623. 06) ng · mL-1 · h, respectively; Cmax were (321.92±85.18) ,(455.83±277.07) ,(319.92±86.07) μg·L-1, respectively; tmax were (0.70±0.45) , (0.50±0.32) ,(0.50± 0.34) h, respectively; t1/2z were (2.78± 1.72) ,(5.29± 2.67) ,(5.31± 2.82) h, respectively. Conclusion The main pharmacokinetic parameters of mangiferin polymorphs in plasma of rats are different, and mangiferin form Ⅴ has the hightest AUC(0-24 h) and Cmax.
