Effect of Disulfiram Combined with Copper on the Proliferation and Migration of Brain Metastases from Lung Cancer
10.3870/j.issn.1004-0781.2019.02.007
- VernacularTitle:双硫仑联合铜对肺癌脑转移癌细胞增殖和迁移的影响
- Author:
Caikui LUO
1
;
Qin TU
;
Xiaoxiang YU
;
Liang MENG
;
Wen FAN
;
Liang TAO
Author Information
1. 武汉大学附属同仁医院(武汉市第三医院) 神经外科
- Keywords:
Disulfiram;
Copper;
Cancer,brain metastases;
Proliferation;
Migration
- From:
Herald of Medicine
2019;38(2):173-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of disulfiram combined with copper (DSF /Cu) on proliferation and migration of brain metastases from lung cancer. Methods The cell were divided into blank control group, valproate group (1.0 mmol·L-1 of valproate) , DSF /Cu (1,2,3,4,5 mg /0.17 mg) . Brain metastases from lung adenocarcinoma were incubated 72 hours.The changes of proliferative ability of cancer cells were detected by CCK-8 method, the effect of DSF /Cu on the migration ability of cancer cells was detected by Transwell chamber, and the degree of apoptosis of cancer cells was detected by Annexus V-PI double staining method. Results The ability of proliferation was significantly inhibited, the ability of migration was significantly reduced,and the rate of cell apoptosis was significantly increased in each dose group of brain metastases from lung adenocarcinoma compared with the blank control group. With the increase of DSF /Cu dose, its ability to inhibit the proliferation and migration of metastases cancer cells and induce apoptosis of metastases cancer cells has increased gradually. Conclusion DSF /Cu can inhibit the proliferation and migration of brain metastases from lung adenocarcinoma and induce apoptosis. DSF is expected to be a new method of the combined treatment of brain metastases cancer.
