Inhibitory effect of Corynebacterium parvum cell wall on bladder cancer cells
10.3760/cma.j.issn.1673-4904.2019.02.001
- VernacularTitle:短棒状杆菌细胞壁对膀胱癌细胞抑制效果的检验
- Author:
Ming SHI
1
;
Qiongwen ZHANG
;
Jing ZHANG
;
Wanxia LI
;
Xiaoguang SHAO
Author Information
1. 大连市妇女儿童医疗中心检验科 116037
- Keywords:
Cell wall;
Urinary bladder neoplasms;
Corynebacterium parvm;
Rat
- From:
Chinese Journal of Postgraduates of Medicine
2019;42(2):97-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the inhibitory effect of Corynebacterium cell wall extract on bladder cancer cells. Methods The bladder RNA was extracted from bladder cancer rats, and concentration and purity of RNA was detected. The extracted RNA was reversely transcribed into cDNA, then the primers were designed and the Grim19 gene, β-actin gene, Stat3 gene was amplified. Finaly the PCR product was subjected to agarose gel electrophoresis. Results OD260 was 0.07, and OD260/OD280 was 2.03 in the group of Corynebacterium parvm extract(3-5# ); OD260 was 0.12, and OD260/OD280 was 2.07 in the group of MNU( 6-5#);OD260 was 0.08, and OD260/OD280 was 2.07 in the group of physiological saline(7-5#) . The results of agarose gelelectrophoresis showed that Grim19 gene and Stat3 gene was expressed highly in the bladder of rats from the group of CP cell wall extract (3-5# ). Grim19 gene was expressed lowly, while Stat3 gene was expressed highly in the bladder of rats from the group of MNU (6-5#). Grim19 gene and Stat3 gene was expressed normally in the bladder of rats from physiological saline(7-5). Conclusions The expression situation of antitumor gene Grim19 and tumor gene Stat3 in the bladder of rats was inhibited by the cell wall extract of Corynebacterium parvm, which indicates that the cell wall extract of Corynebacterium parvm has inhibitory effect on bladder cancer cells.
