p-Coumaric acid ameliorates ethanol-induced kidney injury by inhibiting inflammatory cytokine production and NF-κB signaling in rats
- Author:
Sabitha RAMAKRISHNAN
1
;
Nishi KUMARI
;
Gunasekaran Prasanna VINOTH
;
Annamalai GOVINDHAN
;
Agilan BALUPILLAI
;
Ganeshan MATHAN
Author Information
- Keywords: p-Coumaric acid; Apoptosis; Alcohol; Cytokine; Inflammation; Nephrotoxicity
- From:Asian Pacific Journal of Tropical Biomedicine 2019;9(5):188-195
- CountryChina
- Language:Chinese
- Abstract: Objective: To examine the effects of p-coumaric acid on ethanol-induced kidney injury in Swiss Wistar rats. Methods: Ethanol (25％ v/v) was used to induce nephrotoxicity in rats. p-Coumaric acid was orally administered at 50, 100, or 200 mg/kg body weight. The levels of oxidative parameters were determined; pro-inflammatory biomarkers were analyzed by Western blotting and apoptotic protein was analyzed by immunohistochemistry. Results: Ethanol treated rats showed decreased levels of antioxidants and aberrant production of pro-inflammatory cytokines (IL-6, IL1β, TNF-α), NF-κB activation and imbalance of pro-and anti-apoptotic proteins (Bcl-2, Bax, caspase 3). Meanwhile, p-coumaric acid restored antioxidant levels and decreased the levels of inflammatory cytokines, NF-κB, and pro-apoptotic proteins and increased Bcl-2 expression. Conclusions: p-Coumaric acid ameliorates ethanol-induced kidney injury by restoring antioxidant production and suppressing cellular apoptosis and inhibiting NF-κB expression. p-Coumaric acid should be further investigated as a promising candidate for ethanol-induced kidney toxicity.
