Synergistic promotion of inflammatory chondrocyte reverse differentiation by the combination of curcumin and magnesium sulfate
10.3969/j.issn.2095-4344.1594
- VernacularTitle:姜黄素和硫酸镁联合应用协同促进炎性软骨细胞的逆分化
- Author:
Yang WANG
1
;
Zhuoyue SONG
;
Guangheng LI
Author Information
1. 郑州大学第一附属医院
- Keywords:
Curcumin;
Magnesium;
Chondrocytes;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2019;23(12):1410-1415
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Osteoarthritis is a common cause of pain and disability in the elderly. The underlying cause is the combination of inappropriate mechanical stress, inflammatory mediators and biochemical factors. Curcumin has been shown to have anti-oxidant, anti-inflammatory, anti-bacterial, anti-tumor, neuroprotective, and cardioprotective effects, radiation protection and therapeutic effects on osteoarthritis. Similarly, magnesium sulfate can relieve joint pain and inhibit joint destruction. OBJECTIVE: To investigate the mechanism by which the combined use of curcumin and magnesium sulfate exerts synergistic effect to promote inflammatory chondrocyte reverse differentiation.METHODS: Primary cultured inflammatory chondrocytes were subjected to monolayer culture in vivo. Cell proliferation assay (MTS) was used to detect the proliferation of inflammatory chondrocytes under in vitro monolayer culture conditions. The experimental cells were divided into four groups and underwent 3D induced reverse differentiation culture for 18 days: single culture of chondrocytes (chondrocyte group) , inflammatory chondrocyte cultured with curcumin (curcumin group) , inflammatory chondrocytes cultured with magnesium sulfate (magnesium sulfate group) , and inflammatory chondrocytes cultured with curcumin and magnesium sulfate (combination group). RESULTS AND CONCLUSION: MTS proliferation experiments showed that inflammatory chondrocytes at passage 3 had a higher rate of early proliferation and a lower degree of differentiation. Quantitative PCR results showed that the mRNA levels of type II collagen, proteoglycan and SOX9 were significantly higher in the combination group than in the curcumin group or magnesium sulfate group (P < 0.01). The size of the gross specimens and the positive area of chondrocyte reverse differentiation for alcian blue staining and safranin O staining in the combination group were significantly larger than those in the other three groups (P < 0.01). TUNEL staining results indicated that the positive area of apoptosis-specific staining in the combination group and magnesium sulfate group was significantly smaller than that in the other two groups (P < 0.01). Therefore, the combined use of curcumin and magnesium sulfate has the synergistic effect to promote the reverse differentiation of inflammatory chondrocytes.
