Micro RNA-10b regulates aldehyde dehydrogenase 1 mRNA and protein expression in breast cancer MCF-7 cell line
10.3969/j.issn.2095-4344.1622
- VernacularTitle:微小RNA-10b调控乳腺癌MCF-7细胞中乳腺癌干细胞标记物乙醛脱氢酶1 mRNA和蛋白的表达
- Author:
Mingli HAN
1
;
Pengwei LÜ
;
Xueke QIAN
;
Xue YANG
;
Yunqing YANG
;
Yuanting GU
Author Information
1. 郑州大学第一附属医院乳腺外科
- Keywords:
Breast Neoplasms;
Neoplastic Stem Cells;
MicroRNAs;
Aldehyde Dehydrogenase;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2019;23(12):1349-1353
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: MicroR-10 b can regulate the characteristics of breast cancer stem cells, and acetaldehyde dehydrogenase 1 (ALDH1) is one of the most important markers of breast cancer stem cells. The interaction between them in breast cancer cells needs further explorations. OBJECTIVE: To investigate whether over-expression of microRNA-10 b affects ALDH1 mRNA and protein levels in human breast cancer MCF-7 cells. METHODS: hsa-miR-10 b mimics or its negative control was transfected into breast cancer MCF-7 cell line. At 48 hours after transfection, the mRNA and protein expression of ALDH1 in the cells was detected using real-time RT-PCR and western blot assays, respectively. RESULTS AND CONCLUSION: Over-expression of microR-10 b was found in the MCF-7 cell line transfected with hsa-miR-10 b mimics, which was significantly higher than that in the control group (P=0.003 47). Both of ALDH1 mRNA and protein levels were up-regulated in the MCF-7 cell line overexpressing microR-10 b, as compared with the control group (P=0.009 54 and P=0.003 11, respectively). To conclude, over-expression of microR-10 b induces the ALDH1 mRNA and protein expression in the breast cancer MCF-7 cell line, providing new evidence that microR-10 b regulates the invasion and metastasis of breast cancer cells.
