Biocompatibility of anodic titanium oxide: an experimental research
10.3969/j.issn.2095-4344.1636
- VernacularTitle:阳极氧化钛生物相容性的实验研究
- Author:
Shaoyang LIU
1
;
Hanlin ZOU
Author Information
1. 上海市普陀区中心医院骨科
- From:
Chinese Journal of Tissue Engineering Research
2019;23(10):1575-1580
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Studies have confirmed that titanium dioxide coating can improve the adhesion of implant surface, promote osteoblast proliferation and other cell functions, and improve the bone integration ability between the surrounding bone and implant. But little has been done in the use of titanium dioxide coating as a coating for internal and external fixation to prevent screw loosening. OBJECTIVE: To prepare titanium dioxide coated titanium screws and to observe the effect of titanium dioxide coated titanium screws used for bone fixation to reduce screw loosening. METHODS: Titanium dioxide coating was prepared on the surface of titanium screw by anodic oxidation. Thirty male New Zealand white rabbits (provided by the Central Laboratory of Shanghai Putuo District Central Hospital in China) were randomly divided into two groups (n=15 per group). Titanium dioxide coated screws were implanted in the right tibia of the rabbits in the experimental group, and ordinary titanium screws were implanted in the right tibia of the rabbits in the control group. Bone tissue around the screw canal was taken at 1 day, 1 and 4 weeks after implantation, and levels of platelet-derived growth factor and vascular endothelial growth factor genes were detected using RT-PCR. Four weeks after implantation, bone tissue around the nail canal was taken for hematoxylin-eosin staining. RESULTS AND CONCLUSION: (1) The results of hematoxylin-eosin staining showed that inflammatory cell infiltration was seen in the screw canal of the control group, and some screw canal threads were destroyed and absorbed. In the experimental group, the screw threads were clear and had no damage or absorption; inflammatory cells in the screw canal were less exuded. (2) RT-PCR analysis revealed no significant difference in the expression of platelet-derived growth factor and vascular endothelial growth factor gene between the two groups at 1 day after implantation (P> 0.05), while the expression of platelet-derived growth factor and vascular endothelial growth factor gene in the experimental group was significantly higher than that in the control group at 1 and 4 weeks after implantation (P < 0.05 or P < 0.01). Therefore, titanium dioxide coated titanium screws are more biocompatible than common titanium screws and can effectively prevent screw loosening when used for internal fixation.
