Effects of hypoxia on expression of hypoxia inducible factors 1α and 2α in human lung cancer cell line A-549
10.3969/j.issn.1006-5725.2019.03.010
- VernacularTitle:缺氧对肺癌细胞系A-549表达缺氧诱导因子1α和2α的影响
- Author:
Yonghai FENG
1
;
Hongyun LI
;
Shaoyi MIAO
;
Xiaoshan SHI
;
Wenfei ZHAO
Author Information
1. 郑州大学第五附属医院呼吸与危重症医学科
- Keywords:
lung cancer;
A549;
HIF-1α;
HIF-2α
- From:
The Journal of Practical Medicine
2019;35(3):381-383,387
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of hypoxia-inducible factor (HIF-1α, HIF-2α) expression level in lung cancer A-549 cells under normoxic conditions, different hypoxia durations, and different oxygen concentrations. Methods A549 cells were divided into normoxic group, time control group, and oxygen concentration control group. Western blot was used to detect the expression of HIF-1α and HIF-2α in A-549 cells.Results The expression of HIF-1α and HIF-2α protein were lower under normoxia and significantly increased under hypoxic conditions. The difference was statistically significant. The lower the oxygen concentration, the more HIF-1α and HIF-2α protein expression levels were. The differences between high and high were statistically significant. The expression of HIF-1α protein increased at 2 h after hypoxia, peaked at 8 h, appeared plateau at 8 to 16 h, and decreased at 32 h, with a statistically significant difference. HIF-2α proteins gradually increased with prolonged hypoxia. Conclusions Under hypoxic conditions, the expression of HIF-1α and HIF-2α are increased, and the expression of HIF-2α has a time-dependent pattern, which may have more important biological significance.
