Long non-coding RNA XLOC009038 promotes proliferation, migration and invasion of esophageal squamous cell carcinoma
10.3969/j.issn.1006-5725.2019.03.008
- VernacularTitle:长链非编码RNA XLOC009038促进食管鳞癌细胞增殖、迁移和侵袭
- Author:
Xin DING
1
;
Yong ZHENG
;
Jing LI
;
Xue ZHENG
;
Juanjuan TONG
;
Danping YANG
;
Weigang CHEN
Author Information
1. 石河子大学医学院第一附属医院
- Keywords:
esophageal carcinoma;
long non-coding RNA;
XLOC_009038;
caspase3
- From:
The Journal of Practical Medicine
2019;35(3):369-374
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of long-chain non-coding RNA XLOC009038 on the proliferation, apoptosis, migration and invasion of esophageal squamous cell carcinoma EC 109 and EC9706 cells and explore its mechanism. Methods XLOC009038 interfering plasmid was constructed and transfected into EC 109 and EC9706 cells to down-regulate the expression of XLOC009038 gene. MTT colorimetry and clonogenic assay were used to observe the changes of cell proliferation and cloning ability before and after gene down-regulation.Flow cytometry was used to detect apoptosis. Transwell was used to measure the changes of cell migration and invasion ability before and after transfection. Western blot was used to detect the expression of procaspase 3 protein in cells before and after transfection. Results The expression of XLOC009038 gene in the two cells was significantly lower than that in the control group (P < 0.001). After down-regulation of XLOC009038 gene expression, the cloning and proliferation ability of EC 109 and EC9706 cells decreased significantly (P< 0.05). Compared with the control group, the migration and invasion ability of EC 109 and EC9706 cells decreased significantly (P < 0.001).Flow cytometry showed that the apoptosis rate of EC 109 and EC9706 cells increased after down-regulation of XLOC009038 (P <0.001). The expression of procaspase 3 increased in the experimental group after interfering with XLOC009038 (P = 0.013; P < 0.001). Conclusions Over-expression of XLOC009038 might be closely related to occurrence and development of the esophageal cancer. Over-expression of XLOC009038 can enhance the proliferation, apoptosis, migration and invasion of esophageal cancer cells in vitro through the procaspase3 pathway.
