Changes of TGFβ/Smad signaling expression in oral squamous cell carcinoma after hyperthermia

VernacularTitle:TGFβ/Smad信号通路在口腔鳞癌热疗中的表达变化
Author: Hao ZHOU ¹ ; Yi LIU ; Chuhang LIAO ; Jun GUO ; Wei FEI
Author Information

1. 四川省医学科学院·四川省人民医院口腔科
Keywords: Hyperthermia; Oral squamous cell carcinoma (OSCC); TGFβ/Smad signaling
From: Journal of Practical Stomatology 2019;35(1):24-27
CountryChina
Language:Chinese
Abstract: Objective: To study the changes of TGFβ/Smad signaling expression in oral squamous cell carcinoma (OSCC) after hyperthermia. Methods: The expression of TGFβ, Smad2, 3, 4 and 7 in OSCC of tumor bearing nude mice were examined by quantitative real-time RT-PCR and Western blot respectively. Results: The mRNA expression of TGFβ, Smad2 and 3 was decreased significantly, and the mRNA levels of Smad7 was elevated in hyperthermia (HT) group (n = 10) (P ＜ 0. 05), but the mRNA expression of Smad4 did not change significantly compared with that of control group (n = 10) . The changes of protein expression of TGFβ, Smad7 and 4 were consistent with that of mRNA, the expression of Smad2 and 3 was not significantly different between the groups, but the expression of p Smad2 and p Smad3 decreased dramatically in HT group (P ＜ 0. 05) . Conclusion: TGFβ/Smad signaling exhibits important role in the antitumor effects of hyperthermia, and the inhibition of Smad7 on R-Smad phosphorylation may play a key role.