The inhibitory effect of ozone water on bad breath pathogens in vitro
10.3969/j.issn.1001-3733.2019.01.004
- VernacularTitle:臭氧水对口臭相关细菌的体外抑制效果研究
- Author:
Jing JIANG
1
;
Chunhui LI
;
Minhai NIE
Author Information
1. 西南医科大学口腔医学院
- Keywords:
Bad breath;
Porphyromonas gingivalis (Pg);
Prevotella intermedia (Pi);
Fusobacterium nucleatum (Fn);
Ozone water
- From:
Journal of Practical Stomatology
2019;35(1):20-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the inhibitory effect of ozone water on bad breath pathogens in vitro. Methods: In vitro cultured bad breath pathogens Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi) and Fusobacterium nucleatum (Fn) were identified by Gram stain and a PCR test. Ozonated water by was prepared by ozone generator and the concentration of ozone in water was measured using iodine titration. Artificial saliva was used to observe its interference on ozone function. Results: Gram stain and PCR results were consisted with strain characteristics. The ozone concentration of ozonated water reached to the maximum of 0. 2 mg/L. Ozone water with the concentration was 0. 05, 0. 1 and 0. 2 mg/L inhibited the proliferation of Pg, Pi and Fn. But the inhibitory effect was weakened when the concentration decreased. The artificial saliva reduced the effect of ozone water. Conclusion: The Pg, Pi and Fn can be inhibited by ozone water. Artificial saliva may reduce the effects of ozone water on the bacteria.
