A novel tissue engineering complex——BMSCs Sheet-RADA16 scaffold constructed by cell sheet and self-assembled peptides technique
10.3969/j.issn.1001-3733.2019.01.001
- VernacularTitle:联合细胞膜片和自组装多肽技术构建一种新型BMSCs膜片-RADA16组织工程复合体的研究
- Author:
Doudou LI
1
;
Weiwei ZHOU
;
Lei WANG
;
Lu LIU
;
Chunrong LI
;
Silin LIU
;
Meng CAO
Author Information
1. 军事口腔医学国家重点实验室
- Keywords:
Bone marrow mesenchymal stem cells (BMSCs);
Cell sheet;
Self-assembled peptides;
Tissue engineering
- From:
Journal of Practical Stomatology
2019;35(1):5-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct a novel tissue engineering complex, BMSCs sheet-RADA16 scaffold, by combining cell sheet and self-assembled peptides.. Methods: The self-assembled peptide RADA16 scaffold was wrapped with the BMSCs cell sheet. The morphology of the cells and the complex were observed by SEM and confocal laser microscopy, and the proliferation of cells was assessed by CCK-8. The osteogenic differentiation of BMSCs was examined by detection of related gene expression with RT-PCR. Results: Compared with the BMSCs cell sheet, the numbers of cells on RADA16 scaffold growth rapidly at 3 rd-8 th day, and BMSCs were more on the scaffold than those on the cell sheet (P＜0. 05) . RT-PCR results showed that the expression level of osteogenesis-related genes was higher in the complex (P＜0. 05) . Conclusion: BMSCs Sheet-RADA16 Scaffold may promote proliferation and osteogenic differentiation of BMSCs.
