circ_0074930 regulates radiosensitivity of colorectal cancer cells by targeting miR-1238
10.3760/cma.j.issn.1674-6090.2019.01.019
- VernacularTitle:circ_0074930通过靶向miR-1238调控结直肠癌细胞放疗敏感性
- Author:
Kun YI
1
;
Hui YANG
;
Hong PENG
Author Information
1. 南充市中心医院影像科 637000
- Keywords:
Circular RNA;
Colorectal cancer;
Radiosensitivity
- From:
Chinese Journal of Endocrine Surgery
2019;13(1):81-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between circ_0074930 and radiosensitivity of colorectal cancer,as well as its molecular mechanism.Methods Colorectal cancer cell lines HT29,SW480,SW620,LOVO and HCT116 were irradiated with different radiation doses.Radiosensitivity of these 5 cells lines was detected through survival fraction at 2 Gy (SF2 value).High-throughput circRNA chip was used to screen circRNA gene between HT29 and HCT116.The effect of siRNA,designed to target circ_0074930,on the proliferation and radio sensitivity of HT29 cell line was observed.Bioinformatics prediction software was used in circ_0074930 target miRNA predictive analysis and luciferase reporter system was employed in circ_0074930 target miRNA verification.The effects of inhibition and overexpression of miR-1238 on proliferation and radiosensitivity of HT29 cells were observed.Results The radiosensitivity order of these 5 cell lines from low to high(SF2 value from high to low) was HT29(0.81±0.02),SW480(0.70±0.03),SW620(0.62±0.03),Lovo(0.51±0.02)and HCT116 (0.42±0.03).Expression levels of circ_0074930 in HT29 cell line was 7.6 times than in HCT116 cell line.circ_0074930 silence by small interfering RNA reduced HT29 cell proliferation and enhanced radiosensitivity.miR-1238 was identified and validated to be a target miRNA of circ_0074930.Overexpression of miR-1238 reduced HT29 cell proliferation and enhanced radiosensitivity,but inhibition of miR-1238 expression could promote HT29 cell proliferation and decrease radiosensitivity.Conclusion circ_0074930 decreases radiosensitivity and promote proliferation of HT29 cell through suppressing miR-1238.
