Effect of target silencing FGF8b gene on proliferation and apoptosis of ovarian cancer SKOV-3 cells
10.3760/cma.j.issn.1674-6090.2018.06.016
- VernacularTitle:靶向沉默FGF8b基因对卵巢癌SKOV-3细胞增殖和凋亡的影响
- Author:
Pengli HAN
1
;
Jingmin ZHANG
;
Fengmei LI
Author Information
1. 450007,河南郑州大学附属郑州中心医院转化医学中心
- Keywords:
FGF8b;
Ovarian cancer;
RNA interference;
Cell proliferation;
Cell apoptosis
- From:
Chinese Journal of Endocrine Surgery
2018;12(6):514-517
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of targeted silencing FGF8b gene on the proliferation and apoptosis of ovarian cancer SKOV-3 cells and its possible molecular mechanism.Methods The design and synthesis of small interfering RNA targeting FGF8b (siRNA),was transfected into ovarian cancer cell SKOV-3,using four methyl thiazolyl tetrazolium (MTT) method to detect cell proliferation and apoptosis of TUNEL kit cells.The expression of Bcl-2 and Bax protein by Western blot assay in cells.Results FGF8b siRNA could inhibit the expression of FGF8b protein in ovarian cancer SKOV-3 cells.The experimental group showed a high proportion of TUNEL positive cells(22.33±2.517) (P＜0.01).The down-regulation of FGF8b protein significantly inhibited the proliferation of ovarian cancer SKOV-3 cells,reduced the expression of Bcl-2(0.586±0.025) (P＜0.01),and increased the expression of Bax(0.334±0.019) (P＜0.01).Conclusion Down regulation of FGF8b expression can inhibit the proliferation of ovarian cancer SKOV-3 cells and induce cell apoptosis,which may be related to the expression changes of Bcl-2 and Bax protein,suggesting that FGF8b plays an important role in the development of ovarian cancer.
