The expression and role of Caspase3, Caspase12 and CHOP in pituitary tissues of rats with acute necrotizing pancreatitis
10.3760/cma.j.issn.1674-6090.2018.06.009
- VernacularTitle:Caspase3、Caspase12、CHOP在急性坏死性胰腺炎大鼠垂体组织中的表达及作用
- Author:
Wenhong DENG
1
;
Wenyi GUO
;
Xiaobo HE
;
Fangchao MEI
;
Yupu HONG
;
Xiaojia YANG
;
Weixing WANG
Author Information
1. 430060,武汉大学人民医院普通外科
- Keywords:
Acute necrosis pancreatitis;
Pituitary;
Apoptosis;
Caspase3;
Caspase12;
CHOP
- From:
Chinese Journal of Endocrine Surgery
2018;12(6):478-482
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the pathological changes of pituitary tissue in rats with acute necrotizing pancreatitis and to explore the mechanism of pituitary tissue injury in rats.Methods 24 SD rats were randomly divided into normal group (N group,n=8),sham operation group (SO group,n=8),and acute necrotizing pancreatitis group (ANP group,n=8).ANP model was established by retrograde injection of 5％ sodium taurocholate into the biliopancretic duct.The serum levels of amylase(AMY) and lipase (LIP) were detected by automatic biochemical.The serum levels of growth hormone (GH),adrenocorticotropic hormone (ACTH),thyroid stimulating hormone (TSH) and follicle-stimulating hormone (FSH) were measured by radioimmunoassay.The pathological changes of pancreatic tissue and pituitary tissue were observed by the light microscope.The expression of Casepase3,Caspasel2 and CHOP in pituitary tissue were determined by immunohistochemical method.Results Compared to SO group,the serum levels of AMY(8679.16±307.60) U/L and LIP(9376.83±380.92) U/L were significantly higher in ANP group (P＜0.05).The serum levels of ACTH (0.92±0.41) pg/ml,TSH (0.14±0.06) pg/ml,and FSH (2.01±0.38) pg/ml were significantly lower in ANP group(P＜0.05).The expression of Caspse 3 (65.66±7.58),Caspase12(70.66±4.76) and CHOP(143.16±19.05) in pituitary tissue were significantly increased in ANP group (P＜0.05).The pancreatic injury was more severe in ANP group under light microscope (P＜0.05).The degree of hyperemia of pituitary tissue of ANP group was aggravated.Conclusion Pathological changes occur in rat pituitary tissues and endoplasmic reticulum stress injury plays a role in pituitary injury during ANP.
