Effect of sleeve gastrectomy on the expression of bile acid transporters in the terminal ileum in obese type 2 diabetic rats
10.3760/cma.j.issn.1674-6090.2018.06.004
- VernacularTitle:袖状胃切除术对肥胖型2型糖尿病大鼠末端回肠胆汁酸转运体表达的影响
- Author:
Jiaobao HUANG
1
;
Jixing LIU
;
Jian XU
;
Jiajun YIN
Author Information
1. 116001,大连大学附属中山医院肝胆腹腔镜外科
- Keywords:
Sleeve gastrectomy;
Bile acid;
Bile acid transporter;
Type 2 diabetes
- From:
Chinese Journal of Endocrine Surgery
2018;12(6):454-458
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sleeve gastrectomy (SG) on the distal ileum apical sodium-dependent bile acid transporter (ASBT) and ileum bile acid binding protein (IBABP)in obese type 2 diabetic rats.Methods Thirty 8-week-old male Sprague-Dawley rats were divided into groups O,F and N according to the random number table method.Among them,groups O and F were given high-sugar and high-fat diets,and group N were fed with normal diets before and after surgery.After 1,2 and 4 weeks,the body weight,blood glucose and plasma total bile acid levels were measured.The experimental animals were sacrificed 4 weeksafter operation and the terminal ileum tissue was harvested.Two types of ASBT and IABAP were detected by Western blot and immunohistochemistry.Bile acid transporters were tested.Results Blood glucose in group O was significantly lower than that in group F at 4 weeks postoperatively,(5.8±1.77) and (19.55±2.16) mmol/L,respectively (P＜0.05).Body weight in group O was significantly lower than that in group F at 4 weeks postoperatively,(182±10.66) g and (362±23.54) g,respectively (P＜0.05).Plasma total bile acid levels in group O were significantly higher than those in group F and N at 4 weeks after operation (52±18.48),(6.39±1.18),(5.89±1.21)μmol/L,respectively(P＜0.05).At the end of the 4th postoperative period,the expression of ASBT and IBABP in the distal ileum of group O increased,but there was no significant change in group F and group N.Conclusions The effect of SG on the hypoglycemic and weight-loss of obese T2DM rats is definite.The level of bile acid in plasma increases significantly after SG,and the expression of bile acid transporter in the terminal ileum of rats is one of the mechanisms.
