Expression of miR-4687-5P and STIM1 in esophageal squamous cell carcinoma and their regulation mechanism
10.13315/j.cnki.cjcep.2019.02.002
- VernacularTitle:食管鳞状细胞癌中miR-4687-5P及STIM1的表达及调控机制
- Author:
Yan-Li GUO
1
;
Yun-Feng NIU
;
Liu YANG
;
Gang KUANG
;
Wei GUO
;
Zhi-Ming DONG
;
Su-Peng SHEN
;
Jia LIANG
Author Information
1. 河北医科大学第四医院河北省肿瘤研究所病理研究室
- Keywords:
esophageal neoplasms;
esophageal squamous cell cancer;
miR-4687-5P;
STIM1 gene;
methylation
- From:
Chinese Journal of Clinical and Experimental Pathology
2019;35(2):133-138
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression and the methylation status of miR-4687-5P and STIM1 gene in esophageal squamous cell cancer (ESCC) cell lines and ESCC tissue samples,in order to explore the correlation between miR-4687-5P and STIM1 expression,as well as whether they have a common expression regulation mechanism. Methods The qRTPCR and methylation specific PCR (MSP) methods were applied respectively to examine the expression and methylation of miR-4687-5P and STIM1 genes in ESCC cell lines (TE13, KYSE150,T. Tn) and ESCC samples,and further to analyze their correlation. Results The expression of miR-4687-5P and STIM1 genes in ESCC was significantly decreased,and consistent. The weak expression of miR-4687-5P and STIM1 genes was detected in three ESCC cell lines. After treated with 5-Aza-2'-deoxycytidine (5-Aza-Dc,a demethylation agent) ,the expression levels of these two genes were obviously increased. Meanwhile, the methylation bands were obviously weakened or disappeared. The promoter region of STIM1 gene was hypermethylated in ESCC tissues,and its methylation frequency was correlated with the expression of STIM1 and miR-4687-5P (P < 0. 01) . Conclusion miR-4687-5P and STIM1 genes are down-regulated in esophageal carcinoma,and the expression of miR-4687-5P may be regulated by the promoter of its host gene STIM1,and the hypermethylation may be one of the common mechanisms leading to down-regulatory expression of miR-4687-5P and STIM1 genes in ESCC.
