Comparison of immunohistochemical stain and 14C urea breath test in the diagnosis of helicobacter pylori associated gastritis
10.13315/j.cnki.cjcep.2019.01.011
- VernacularTitle:免疫组化染色与14C尿素呼气试验在幽门螺杆菌相关胃炎诊断中的比较
- Author:
Wei-Song WAN
1
;
Le WANG
;
Yu-Chang HU
;
Yu-Fei LIU
;
Dong-Ping ZHENG
;
Qing-Long HU
Author Information
1. 三峡大学第一临床医学院/宜昌市中心人民医院病理科
- Keywords:
helicobacter pylori;
immunohistochemistry;
14C urea breath test;
retrospective study
- From:
Chinese Journal of Clinical and Experimental Pathology
2019;35(1):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To evaluate the value of immunohistochemical stain (IHC) in the diagnosis of helicobacter pylori (HP) -associated gastritis with gastric biopsy tissue. Method239 cases of gastric biopsy were selected. All patients had14 C urea breath test (UBT) at the time of gastric biopsy. IHC for HP was performed with the biopsy tissues. The results were compared with gold standard results. Gold standard was established according to the Chinese Fourth National Consensus report on HP infection with modification. It was considered positive if two or more of the three test results were positive, including IHC, UBT, and HE. Results 239 gastric biopsy specimens fit the criteria. Compared with the gold standard, the sensitivity and specificity value of IHC stains were 97.3％ and 100.0％ respectively, higher than 91.1％, 81.9％ of 14 C UBT, and 80.4％, 92.9％ of HE stain respectively. The14 C UBT showed 9.6％ false positive rate, and 4.2％ false negative rate. Conclusion IHC is a valuable test in the diagnosis of HP-associated gastritis, and it should be recommended for routine histopathologic diagnostic workup if HP gastritis is clinically suspected or chronic active gastritis is identified in the biopsy tissue.
