Expression of long-chain non-coding RNA-LINC00485 in lung cancer and its effect on proliferation and migration of lung cancer cells
10.13315/j.cnki.cjcep.2019.01.010
- VernacularTitle:长链非编码RNA-LINC00485在肺癌中的表达及对细胞增殖和迁移的影响
- Author:
Xiang-Hui WANG
1
;
Jiang-Ping HUANG
;
Feng-He CUI
;
Hai-Yun QIAN
Author Information
1. 华中科技大学同济医学院附属荆州医院心胸大血管外科
- Keywords:
lung neoplasm;
lncRNA-LINC00485;
proliferatio;
migration;
qRT-PCR
- From:
Chinese Journal of Clinical and Experimental Pathology
2019;35(1):42-46
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To observe the expression of longchain non-coding RNA-LINC00485 (LINC00485) in lung cancer cell lines and tissues, and to investigate its effect on the proliferation and migration of lung cancer cells and its mechanism.Methods Quantitative real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect differential expression of LINC00485 in four lung cancer cell lines (H1975, A549, HCC827, H1299), normal alveolar epithelial cells HPAEPIC, and in 12 cases lung cancer tissues and adjacent tissues. Bioinformatics methods were used to predict the microRNA (miRNA) that LINC00485 may bind and target gene that miRNA may bind. Small interfering RNAs (siRNAs) that target silencing LINC00485 were transfected into HCC827 cells by liposomes.The expression levels of LINK00485, miR-361-5p, and p21 activated protein kinase 2 (PAK2) mRNA were detected by qRTPCR. The expression level of PAK2 protein was detected by Western blot. The cell proliferation ability was measured by MTS assay. Cell scratch assay was used to detect cell migration. Results Compared with normal alveolar epithelium, LINC00485 was highly expressed in lung cancer cell lines (P < 0.05), and the expression level was highest in HCC827 cells. The expression of LINC00485 in lung cancer tissues was higher than that in adjacent tissues (P < 0.01). After down-regulation of LINC00485 expression in HCC827 cells, the expression of miR-361-5p was up-regulated (P < 0.01), the expression of PAK2 mRNA and protein was down-regulated (P < 0.01), the proliferative capacity of HCC827 cells was decreased (P < 0.05), and the ability of cell migration was decreased (P < 0.01).Conclusion The expression of LINC00485 is increased in lung cancer cell lines and tissues. Down-regulation of LINC00485 can inhibit the proliferation and migration of lung cancer HCC827 cells by regulating the expression of miR-361-5p and PAK2 genes.
