Effect of up-regulation of miR-92a on the proliferation and angiogenesis of non-small cell lung cancer
10.13315/j.cnki.cjcep.2019.01.009
- VernacularTitle:下调miR-92a对非小细胞肺癌细胞增殖及血管生成因子表达的影响
- Author:
Jian-Hua TANG
1
;
Ping FENG
;
Zhi-Hua ZHANG
;
Bu WANG
Author Information
1. 河北北方学院附属第一医院 药学部
- Keywords:
lung neoplasm;
non-small cell lung cancer;
A549 cells;
human bronchial epithelial cells;
miR-92a;
cell proliferation;
angiogenesis
- From:
Chinese Journal of Clinical and Experimental Pathology
2019;35(1):38-42
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the effect of down-regulation of miR-92 a on the proliferation and angiogenesis of nonsmall cell lung cancer (NSCLC). Methods Human NSCLC cell A549 was divided into three groups: A549 group (non-transfected A549 cells), sc-siRNA group (A549 cells transfected with sc-siRNA) and miR-92a-siRNA group (A 5 4 9 cells trans-fected with miR-92a-siRNA). The relative expression level of miR-92 a, PTEN and vascular endothelial growth factor (VEGF) in A549 cells and human bronchial epithelial (HBE) cells were detected by RT-PCR and Western blot respectively. The proliferation ability of A549 cells in each group was detected by living cell count and crystal violet staining experiment. Results The relative expression of miR-92 a in A549 cells was significantly higher than that in HBE cells (P < 0.05), the expression level of PTEN protein in A549 cells was significantly lower than that in HBE cells (P < 0.05), and the expression level of VEGF protein was significantly higher than that in HBE cells (P < 0.05).In the miR-92a-siRNA group, the relative expression of miR-92 a decreased (P < 0.05), the expression level of PTEN protein in-creased (P < 0.05), and the expression level of VEGF protein decreased (P < 0.05). The expression levels of PI3 K and Akt in miR-92a-siRNA group decreased (P < 0.05). the number of cells and cell proliferation ability in miR-92a-siRNA group reduced. Conclusion The expression of miR-92 a in NSCLC A549 cells is up-regulated, miR-92 a gene silencing can significantly inhibit cell proliferation and inhibit cell angiogenesis, PTEN and VEGF related PI3K/Akt signaling pathways may play an important role in this process.
