Application of D2-40/CD34-CK cocktail antibodies for colorectal cancer with insufficient lymph node harvest
10.13315/j.cnki.cjcep.2019.01.004
- VernacularTitle:鸡尾酒抗体D2-40/CD34-CK对淋巴结检出数量不足结直肠癌标本的临床病理评价
- Author:
Xian-Hua LIU
1
;
Ying-Hao YU
;
Xing-Feng QI
;
Zai-Zeng WU
;
Shun-Qi HU
;
Xi-Sheng XIONG
;
Juan XIANG
;
Zhi-Yong ZHENG
;
Li-Juan QU
;
Xian-Zong YE
Author Information
1. 联勤保障部队第九〇〇医院病理科/福建医科大学福总临床医学院/厦门大学附属东方医院
- Keywords:
colorectal neoplasm;
D2-40/CD34-CK cocktail antibodies;
lymphovascular invasion;
prognosis;
clinicopathological
- From:
Chinese Journal of Clinical and Experimental Pathology
2019;35(1):14-18
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the value of application of D2-40/CD34-CK cocktail antibodies by double immunohistochemical staining for assessment of lymphovascular invasion (LVI) and to determine its prognostic significance in colorectal cancer with insufficient lymph node harvest. Methods Specimens from 133 cases of colorectal cancer with less than 12 lymph nodes were selected. HE staining and double immunohistochemical staining of the cocktail antibodies were performed to compare the difference of the two methods in screening for LVI. The The relationship between LVI confirmed by cocktail antibody immunohistochemical staining and clinicopathological characteristics and overall survival (OS) of patients was analyzed. Results (1) The detection rates of cocktail antibody double immunohistochemical staining and HE staining for LVI were 42.9％ (57/133) and 21.8％ (29/133) with statistically significant difference (P < 0.001). (2) The presence of LVI confirmed by double staining was significantly associated with Dukes staging, depth of invasion, clinical stages, lymph node metastasis and tumor budding (P < 0.05). (3) The presence of LVI, the location and extent of LVI, and the number of tumor cells in thrombus ≥5.5 for cases with LVI ≤2 clusters, were significantly associated with OS (P < 0.05). Conclusion D2-40/CD34-CK cocktail antibodies double staining is superior to routine HE staining in assessing LVI. LVI is intimately associated with tumor stage, lymph nodes metastasis and tumor budding, and it is an independent prognostic factor for CRC patients. It should be a supplementary examination for these patients with insufficient lymph node harvest.
