Sevoflurane reduces cerebral injuries in rats resuscitated from cardiac arrest through down regulating the expression of Bax and Bak
10.3760/cma.j.issn.1671-0282.2019.05.010
- VernacularTitle:七氟醚通过调节Bax和Bak表达减轻心脏骤停大鼠脑损伤的实验研究
- Author:
Hengjie LI
1
;
Hui MAO
;
Yong NAN
;
Hongyan WEI
;
Gang DAI
;
Yan YANG
;
Bo LI
;
Xiaoxing LIAO
Author Information
1. 浙江省人民医院
- Keywords:
Cardiac arrest;
Sevoflurane;
Apoptosis;
Mitochondrial permeability transition pore;
Bax;
Bak
- From:
Chinese Journal of Emergency Medicine
2019;28(5):579-583
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neuroprotective mechanism of sevoflurane in rats resuscitated from cardiac arrest (CA).Methods A ventricular fibrillation-induced CA model was established.Forty Wistar rats were randomly divided into the sham group,sevoflurane group and control group.Apoptosis-related proteins were measured by Western blot at 24 h after restoration of spontaneous circulation (ROSC).The status of mitochondrial permeability transition pore (MPTP) were measured using a spectrophotometer,and the mitochondrial membrane potential (MMP) were measured with JC-1 fluorescent probe.At 72 h after ROSC,the apoptotic index of neurons in hippocampal CA1 region was counted by TUNEL staining.Results The protein expression of Bax,Bak,cleaved-caspase 9,cleavedcaspase 3 and cytosolic cytochrome c were lower in the sevoflurane group (all P＜0.05),the protein expression of Bcl-2 was higher in the sevoflurane group compared with the control group (P＜0.05).The sevoflurane group had a less opening status of MPTP and a higher MMP compared with the control group (all P＜0.05).The sevoflurane group had less apoptotic neurons compared with the control group (P＜0.05).Conclusion By up-regulating the expression of Bcl-2,down-regulating Bax and Bak,sevoflurane could reduce the apoptosis of neurons and decrease the opening of MPTP,eventually reduce cerebral injuries.
