S1PR3 agonist RY-15 promotes bacterial clearance
10.3760/cma.j.issn.1671-0282.2018.10.011
- VernacularTitle:S1PR3特异性激动剂RY-15促进细菌清除的作用研究
- Author:
Jun WANG
1
,
2
,
3
;
Ruyi LEI
;
Jinchao HOU
;
Jungang ZHENG
;
Xiangming FANG
Author Information
1. 310003 杭州,浙江大学医学院附属第一医院麻醉科
2. 宁波市第一医院麻醉科
3. 315010 宁波,宁波市第一医院麻醉科
- Keywords:
Sphingosine 1-phosphate Receptor 3;
Macrophage;
RY-15;
Bacterial clearance
- From:
Chinese Journal of Emergency Medicine
2018;27(10):1117-1120
- CountryChina
- Language:Chinese
-
Abstract:
Objective RY-15, a specific agonist of Sphingosine 1-phosphate Receptor 3, was synthesized for investigating the function and mechanism of S1PR3 in bacterial clearance. Methods Measure the ability of RY-15 with FITC to enter the THP-1 cell after coculture for 5 min, 10 min, 20 min, 30 min through confocal microscopy. The function of GY-5 and RY-15 in bacterial clearance was observed by gentamicin protection test. The phosphorylation level of ERK and p-ERK in THP-1 cell was detected by Western Blot after GY-5 and RY-15 stimulation for different times. Results According to confocal microscopy, RY-15 started to enter the THP-1 cell after stimulating for 10 min and the effect of entering cell was very obvious after stimulating for 30 min. Compared to GY-5 group, live bacteria in the macrophage were largely decreased in the RY-15 group( P<0.05). Conmpared to GY-5 group, the p-ERK level raised largely at different poins. Conclusions RY-15, a specific agonist of Sphingosine 1-phosphate Receptor 3, can promote bacterial clearance through entering cell and the phosphorylation level of ERK is a possible mechanism.
