The Protective effect of oleanolic acid on acute cholestatic liver injury in rats
10.3760/cma.j.issn.1671-0282.2018.10.010
- VernacularTitle:齐墩果酸对大鼠急性胆汁淤积肝损伤的保护作用
- Author:
Yuhong WANG
1
;
Ming SUN
;
Xia CHENG
;
Yuanbo ZHANG
;
Yuzhen ZHANG
;
Mudan FENG
;
Gaosu ZHOU
Author Information
1. 中国人民解放军陆军总医院急诊科
- Keywords:
Oleanolic acid;
Cholestasis;
MRP4;
Nuclear receptors
- From:
Chinese Journal of Emergency Medicine
2018;27(10):1112-1116
- CountryChina
- Language:Chinese
-
Abstract:
Objective The protective effect of oleanolic acid on acute cholestatic liver injury in rats. Methods Thirty male SD rats were randomly divided into 3 groups ( n= 10 per group): sham group, bile duct ligated (BDL) group, and bile duct ligated with oleanolic acid (BDL+OA ) group. After 7 days, liver samples in all rats were collected. Expressions of bile acids pump and nuclear receptors at mRNA and protein levels were detected by RT- qPCR and Western blotting. Results At mRNA level, the expression of Mrp4 and Oatp1 expression in the BDL and BDL+OA groups were increased as compared with that in the sham group. The expression of Mrp4 increased 1.8 times in the BDL group and increased 2.3 times in the BDL+OA group (P<0.05), but the expression of Oatp1 was not statistically significant; AhR was increased 1.7-fold in the BDL group and 2.8 times in the BDL+OA group, Nrf2 was increased 1.5-fold in the BDL group and 2.1 times in the BDL+OA group with statistically significant difference. At the protein level, in the BDL group, Mrp4 expression increased 1.3 times, Oatp1 expression increased 1.5 times, AhR expression increased 1.3 times, Nrf2 expression increased 1.4 fold; in the BDL+OA group, Mrp4 expression increased 1.8 fold, AhR expression increased 1.9 fold, with statistical significance between the two groups. Oatp1 expression increased 1.4-fold in the BDL+OA group as compared with the BDL group showing no statistical significance. Conclusions Oleanolic acid stimulates the hepatic expression of bile acids pump Mrp4 associated with the activation of nuclear receptors AhR and Nrf2 in acute bile ductligated rats.
