The expression and significance of iNOS, PDGF-B and LPS in rat models with Budd-Chiari syndrome
10.3969/j.issn.1008-794X.2019.03.012
- VernacularTitle:诱导型一氧化氮合酶、血小板衍生生长因子-B和脂多糖在布-加综合征大鼠模型中的表达及意义
- Author:
Nan ZHU
1
,
2
;
Tiantian ZHANG
;
Weifu LÜ
;
Delei CHENG
;
Guangya ZHOU
;
Weishi CHEN
;
Baolai LIU
;
Jiangtao REN
;
Dong LU
;
Chunze ZHOU
Author Information
1. 中国科学技术大学附属第一医院介入放射科 230001 合肥
2. 安徽省五河县人民医院影像科
- Keywords:
Budd-Chiari syndrome;
hepatic fibrosis;
inducible nitric oxide synthase;
platelet derived growth factor;
lipopolysaccharide
- From:
Journal of Interventional Radiology
2019;28(3):262-267
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of inducible nitric oxide synthase (iNOS), platelet-derived growth factor (PDGF)-B and lipopolysaccharide (LPS) in rat models with Budd-Chiari syndrome (BCS) . Methods BCS model was established by partial ligation of inferior vena cava in the posterior segment of the liver. The experimental rats were divided into control group (n=20), model group (n=20) and sham group (n=20) . Liver tissues were collected for immunohistochemistry, HE and Masson staining, and the expression levels of iNOS, PDGF-B and LPS were determined. Results The LPS value in model group was higher than that in both control group and sham group (P=0.001) . The mRNA and protein expressions of iNOS and PDGF-B in model group were higher than those in both control group and sham group (P=0.001) . Statistically significant differences in mRNA and protein expressions of iNOS and PDGF-B existed between each other among the subgroups (P=0.001) . In model group iNOS was positively correlated with PDGF-B and LPS; liver fibrosis was positively correlated with LPS and negatively correlated with PDGFB. Conclusion The damage and repair of BCS is a complicated process. The iNOS, PDGF-B and LPS may play different roles in different stages of BCS. How to regulate their balance in liver fibrosis may be a direction that deserves further study.
