Simultaneous determination of six kinds of components in Buyang-Huanwu decoction by UHPLC-MS/MS
10.3760/cma.j.issn.1673-4246.2019.02.017
- VernacularTitle:超高效液相色谱-串联质谱法同时测定补阳还五汤中6种成分含量
- Author:
Lu WANG
1
;
Ningfeng ZONG
;
Man JIANG
;
Chuang LIU
;
Taiping YONG
Author Information
1. 东部战区空军医院药学部
- Keywords:
Bu Yang Huan Wu Tang;
UHPLC-MS/MS;
Amygdalin;
Paeoniflorin;
Ferulic acid;
Calycosin glucosidase;
Quercetin;
Formononetin;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2019;41(2):177-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop the UHPLC-MS/MS method for the determination of amygdalin, paeoniflorin, ferulic acid, calycosin glucosidase, quercetin and formononetin in Buyang-Huanwu decoction. Methods Isocratic elution was carried out with mobile phase consisting of methanol- 2 mM ammonium formate. The separation was performed on Agilent ZORBAX SB-C18 maintained at 35 ℃. The flow rate was 200 μl/min, and the injection volume was 2 μl. The mass spectrometer was operated in the positive and negative ionization electrospray (ESI) mode using multiple monitoring (MRM) for analysis of six components. The mass spectrometric conditions were that ion source temperature 400 ℃, dry gas flow 500 L/h, atomization gas flow rate 75.8 Kpa, spray voltage 4000 V, dry gas temperature 400 ℃. Results The amygdalin, paeoniflorin, ferulic acid, calycosin glucosidase, quercetin and formononetin were all analyzed exactly, and the linear ranges were 0.5-32, 0.2-12.8, 0.1-6.4, 0.8-51.2, 0.4-25.6, 0.08-5.12 ng, respectively. The r were 0.9921, 0.9945, 0.9928, 0.9958, 0.9947, 0.9966, respectively. The recoveries of six analytes ranged from 99.21％ to 101.44％ and the relative standard deviations were all below 2.05％. Conclusions A sensitive, accuracy and suitable UHPLC-MS/MS method has been developed, and the method could be applied for the determination of amygdalin, paeoniflorin, ferulic acid, calycosin glucosidase, quercetin and formononetin in Buyang-Huanwu decoction.
