Effect of Yanghe decoction containing serum on expression of iNOS, COX-2 and NF-κB in breast cancer MCF-7 cells
10.3760/cma.j.issn.1673-4246.2019.02.016
- VernacularTitle:阳和汤含药血清对乳腺癌MCF-7细胞iNOS、COX-2及NF-κB表达的影响
- Author:
Liying LIU
1
,
2
;
Jianwei DOU
;
Gaobiao LI
;
Shuo YANG
;
Zhongbo ZHU
;
Qian HUANG
Author Information
1. 陕西中医药大学西安附属医院外科 710021
2. 西安市中医医院外科 710021
- Keywords:
MCF-7 cells;
Yanghe decoction;
NF-kappa B;
Serum pharmacology(TCD)
- From:
International Journal of Traditional Chinese Medicine
2019;41(2):173-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of iNOS, COX-2 and NF-κB in breast cancer MCF-7 cells treated with Yanghe decoction containing serum. Methods The MCF-7 human breast cancer cells were divided into blank group, low dose group, middle dose group and high dose group (4.5, 9, 18 g/kg, respectively). Real-time RT-PCR was used to detect the mRNA expression of iNOS and COX-2 at 24, 48, 72 and 96 hours, and Western-blot was used to detect the expression of NF-κB protein. Results Compared with the blank group, the expression of iNOS and COX-2 mRNA in the low dose group, middle dose group and high dose group decreased significantly at 24, 48, 72 and 96 hours(P<0.05), the the expression of NF-κB protein in the low dose group, middle dose group and high dose group decreased significantly at 24, 48, 72 and 96 hours (P<0.05). So it reduced with the increase of drug concentration and time. There was no significant difference in 72 hours after intervention. Conclusions The Yanghe decoction could reduce the expression of NF-κB, and then reducing the related inflammatory factors COX-2 and iNOS.
