Impacts of sulforaphane on the Wnt/β-catenin pathway of cultured retinoblastoma cells
10.3760/cma.j.issn.1673-4246.2019.02.015
- VernacularTitle:萝卜硫素对视网膜母细胞瘤细胞Wnt/β-catenin信号通路的影响
- Author:
Shanshan MIAO
1
;
Xiangchun HUANG
Author Information
1. 湖北省恩施土家族苗族自治州中心医院眼科中心 445000
- Keywords:
Retinoblastoma;
Cell proliferation;
Apoptosis;
Wnt signaling pathway;
beta Catenin;
Sulforaphane
- From:
International Journal of Traditional Chinese Medicine
2019;41(2):169-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the impacts of sulforaphane on proliferation, apoptosis and Wnt/β-catenin pathway of cultured retinoblastoma cells. Methods Retinoblastoma Y79 cells were cultured with varying concentrations of sulforaphane (10, 20, 40 mg/L) for 24 and 48 h. Cell proliferation and apoptosis were measured by CCK8 assay and flow cytometry. Apoptosis related proteins Bax and Bcl-2, as well as the expression levels of related proteins in Wnt/β-catenin signaling pathway were determined using Western Blot. Results Sulforaphane suppressed cell proliferation and stimulate apoptosis (12.47％ ± 2.24％, 24.63％ ± 3.44％, 57.49％ ± 5.41％ vs. 3.47％ ± 0.74％) in a dose- and time-dependent manner, respectively. Furthermore, 40 mg/L of sulforaphane increased the expression of Bax (0.57 ± 0.03 vs. 0.12 ± 0.01), but decreased the Bcl-2 (0.16 ± 0.01 vs. 0.81 ± 0.03) protein expression. The results of Western Blot displayed that sulforaphane decreased the expression levels of Wnt (0.19 ± 0.01 vs. 0.74 ± 0.02), β-catenin protein (0.22 ± 0.02 vs. 0.82 ± 0.03), indicating that sulforaphane can inhibit the activation of Wnt/β-catenin signaling pathway. Conclusions The sulforaphane could inhibit the proliferation and induce the apoptosis of retinoblastoma Y79 cells, probably regulating by the Wnt/β-catenin signaling pathway.
