Melatonin attenuates cerebral ischemia-reperfusion injury in mice by inhibiting endoplasmic reticulum stress
10.3760/cma.j.issn.1673-4165.2018.12.007
- VernacularTitle:褪黑素通过抑制内质网应激减轻小鼠脑缺血再灌注损伤
- Author:
Wei HU
1
;
Chongxi FAN
;
Jianjun LYU
;
Zhiqiang MA
;
Tian LI
;
Shuai JIANG
;
Chao DENG
Author Information
1. 第四军医大学生物医学工程系
- Keywords:
Brain Ischemia;
Reperfusion Injury;
Melatonin;
Oxidative Stress;
Endoplasmic Reticulum Stress;
Transcription Factor CHOP;
Neuroprotective Agents;
Disease Models,Animal;
Mice
- From:
International Journal of Cerebrovascular Diseases
2018;26(12):912-916
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of melatonin against cerebral ischemia reperfusion injury (CIRI) in mice and its mechanism.Methods Thirty male C57BL/6 mice were randomly divided into sham operation group,CIRI group,and melatonin treatment group (n =10 in each group).A middle cerebral artery occlusion model was induced by suture method.The degree of brain injury was evaluated by neurological function score,brain water content,and cerebral infarction volume.Western blot analysis was used to detect apoptosis-related proteins Bim,Bcl-2,and endoplasmic reticuhm stress-related molecules C/ EBP homologous protein (C/EBP) expression.Results Compared with the CIRI group,the neurological function score was significantly improved,the degree of cerebral edema was significantly reduced,and the volume of cerebral infarction was significantly reduced in the melatonin treatment group (all P ＜0.05).In addition,the expression of Bcl-2 was significantly up-regulated in the melatonin treatment group,and the expression of Bim and CHOP was significantly down-regulated (all P ＜ 0.05).Conclusion Melatonin may play an anti-CIRI role by regulating CHOP,and endoplasmic reticulum stress plays an important role in CIRI.
