Pre-column derivation method and evaluation of agmatine
10.3969/j.issn.1673-4130.2019.02.030
- VernacularTitle:胍丁胺的柱前衍生方法及评价
- Author:
Juan ZHOU
1
;
Huijing ZHANG
;
Lanlan HU
Author Information
1. 陆军军医大学第二附属医院临床药理基地
- Keywords:
agmatine;
pre-column derivatization;
molecular weight;
quantitative analysis
- From:
International Journal of Laboratory Medicine
2019;40(2):242-246
- CountryChina
- Language:Chinese
-
Abstract:
Agmatine is an endogenous amine synthesized from the decarboxylation of arginine.It has a rich biological effects and presents in plants, bacteria and mammalian tissues.Agmatine is highly polar and has a low molecular weight.There are no UV and fluorescent absorption groups in agmatine structure, so it is difficult to gasify.In addition, the content of endogenous agmatine is low, and there are many interference components in biological samples, and the general method is difficult to detect.The pre-column derivatization of agmatine with fluorescence reagents not only increase the molecular weight of agmatine, but also make them with fluorescence, which greatly improve the detection sensitivity and enable the endogenous agmatine to be well separated.It is an important method to determine the content of agmatine.In this paper, several kinds of precolumn derivatives are summarized, and the advantages and disadvantages of various derivatives, the best derivation conditions and detection methods were also analyzed comprehensively.The aim is to determine the content of agmatine and to provide methods and ideas for its biological research.
