Performance evaluation of the hook effect of five immunoturbidimetric kits for the detection of specific proteins
10.3969/j.issn.1673-4130.2019.02.004
- VernacularTitle:5种免疫透射比浊法试剂盒检测特定蛋白钩状效应的性能评估
- Author:
Jie YANG
1
;
Ling LI
;
Hanyun ZHANG
;
Peng ZHANG
;
Fangyin ZENG
Author Information
1. 南方医科大学南方医院检验科
- Keywords:
immunoturbidimetry;
hook effect;
antigen excess;
safety range
- From:
International Journal of Laboratory Medicine
2019;40(2):140-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the performance of hook effect of five immunoturbidimetric kits for the detection of specific proteins on biochemical analyzers.Methods Five immunoturbidimetric kits with higher market share that came from Beijing BSBE (A), Sichuan maccura (B), Shenzhen Mindray (C), Ningbo Medical System (D) and Beijing Leadman (E) were used to determine six specific proteins.A series of concentration gradient samples were prepared and tested to compare the performance of hook effect from different manufactures′kits when the analytical measurement ranges were known.Results In the five kits, the upper limits of the safe range of antigen excess about ASO, hs-CRP andβ2-MG were relatively higher in B and C.No hook effect occurred at the approximate concentration of 10 000IU/mL, 1 000mg/L and 226mg/L respectively.The highest upper limits for CysC were C and E kits, and both were greater than 112mg/L.The upper limits of the safety range for other manufacturers were more than 700mg/L about RBP except for D.The maximum upper limit of mALB was D.Hook effect did not appear at the concentration of 43 560mg/L approximately.Conclusion Different manufactures′immunoturbidimetric kits have different hook effect performance.The laboratories should verify the hook effect performance before using the kits, and select the most suitable kit to prevent hook effect.
