Optimization of experimental conditions for preparation of rat and mouse platelet rich plasma by single centrifugation
10.3969/j.issn.1673-4130.2019.02.001
- VernacularTitle:一次离心法制备大、小鼠富血小板血浆实验条件的优化
- Author:
Zhenwei SUN
1
;
Baocheng CANG
;
Qun NING
;
Jingxiang ZHAO
;
Penglong LI
Author Information
1. 解放军第153中心医院临检中心
- Keywords:
rat;
mouse;
platelet rich plasma;
centrifugal condition
- From:
International Journal of Laboratory Medicine
2019;40(2):129-131,135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the optimal centrifugation conditions for preparation of rat and mouse platelet rich plasma (PRP) by single centrifugation.Methods Arterial blood of rats and mice by femoral artery cannulation and cardiac puncture were obtained respectively, anticoagulation with 14％CPDA-1, while white blood cells in the blood were filtered out.Then the blood was divided into sterile EP tubes, while PRP was prepared by centrifugation in different conditions (the centrifugal force was 300×g-600×g, and the centrifugal time was 4-12min).The number of blood cells of the anticoagulant whole blood, the leukocyte-depleted blood sample and PRP were counted by hematology analyzer, and platelet recovery rates were compared between different methods.Results The platelet recovery rate was highest when the blood samples of rats and mice were centrifuged at 400×g and 300×g for 8min respectively.Conclusion It is a key to prepare PRP by single centrifugation that selecting the appropriate centrifugal force and time and reaching a critical state before the formation of the buffy coat.
