Quantification and Genotyping of Trace Samples
- VernacularTitle:微量生物检材定量及分型
- Author:
Li SONG
1
;
Song LIU
;
Hui WU
;
Shao-Ping FANG
;
Yan-Fang FU
Author Information
1. 杭州市公安局西湖区分局刑事科学技术室
- Keywords:
forensic genetics;
tandem repeat sequences;
real-time fluorescence quantification;
trace samples
- From:
Journal of Forensic Medicine
2018;34(6):656-658
- CountryChina
- Language:Chinese
-
Abstract:
Objective To introduce real-time polymerase chain reaction (real-time PCR) into the initial sample screening, to improve the effectiveness of traditional trace sample extraction method.Methods Serial diluted 9947A was quantified using a Rotor-Gene Q real-time RT-PCR, and the genotype was determined with AmpF?STRTMIdentifilerTMPlus PCR kit.Thus a quantitative threshold model was built to obtain complete STR typing from the trace samples.In addition, 903 trace samples were used to verify the reliability.Results When the samples quality concentration was＞0.03 ng/μL, the effective STR typing could be directly obtained;when the concentration was＞0.01 and≤0.03 ng/μL, the effective STR typing could be directly obtained by optimizing the PCR thermal cycle parameters (30 cycles);and when the concentration was≤0.01 ng/μL, no effective map could be obtained even if PCR was optimized.Conclusion The real-time PCR quantitative threshold model is effective for the screening of trace samples.
