Determination of Oleandrin in Blood and Liver Samples by LC-MS/MS
- VernacularTitle:LC-MS/MS测定血液、肝组织中的欧夹竹桃苷
- Author:
Jin-Xiao ZHAI
1
;
Hui YAN
;
Min SHEN
;
Bao-Hua SHEN
;
Wei LIU
Author Information
1. 司法鉴定科学研究院 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台
- Keywords:
forensic toxicology;
poisoning;
blood;
liver;
liquid chromatography-tandem mass spectrometry;
oleandrin
- From:
Journal of Forensic Medicine
2018;34(6):585-589
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical method for the determination of oleandrin in blood and liver tissues, which could be applied to the cases of death caused by oleander poisoning.Methods Blood or liver tissues underwent a liquidliquid extraction (LLE) using ethyl acetate, and the extract was separated on an Agilent ZORBAX SB-C18column and eluted with a gradient of acetonitrile and 20 mmol/L ammonium acetate (containing0.1％formic acid).Oleandrin was detected using electrospray positive ionization (ESI+) with multiplereaction monitoring (MRM) mode.Results Oleandrin showed excellent linearity in both blood and liver samples in the corresponding linear range (r＞0.995 0), with detection limits 1 ng/mL and 2 ng/g, respectively, extraction recovery rates greater than 70.50％, both intra-and inter-day precisions less than10.71％, accuracies 98.42％-111.63％, and matrix effects 91.52％-106.39％.The method was successfully applied to a case of suspected oleander poisoning.Oleandrin was detected in the blood, urine, liver tissues, bile, stomach wall tissues and stomach contents of the cadaver, with the content ranging from65.5 to 29 600.0 ng/mL (ng/g).Conclusion The method developed in this study is simple and convenient to operate with good selectivity, and is suitable for the analysis of oleandrin in biological samples such as blood and liver tissues, which can provide technical support for forensic identification and clinical diagnosis and treatment of oleander poisoning.
