Expression of NKCC1 and Na-K-ATPase in C57BL/6J mice with age-related hearing loss
10.16066/j.1672-7002.2019.02.004
- VernacularTitle:Na-K-2Cl联合转运子1与钠钾交换ATP酶在C57BL/6J小鼠中的表达
- Author:
Biru ZHANG
1
;
Hanqing LIN
;
Yongming CHEN
;
Yiqing ZHENG
;
Guidi LI
;
Qiuping LU
;
Haidi YANG
Author Information
1. 南方医科大学顺德医院耳鼻咽喉头颈外科
- Keywords:
Animal Experimentation;
Presbycusis;
Sodium-Potassium-Exchanging ATPase;
Potassium Channels
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2019;26(2):71-73
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE Disturbance of K+ ion balance in inner ear is associated in age-related hearing loss. Our study is to investigate the role of NKCC1 and Na-K-ATPase in cochlea and auditory function regulated by with different expression of NKCC1 and Na-K-ATPase. METHODS Auditory threshold of young or old C57BL/6J mice was measured by auditory brainstem response(ABR). The expression of NKCC1 and Na-K-ATPase in mice cochlea were evaluated by reverse transcription polymerase chain reaction(RT-PCR) and western blotting. Furosemide and Ouabain were applied in vivo to inhibit NKCC1 and Na-K-ATPase in C57BL/6J mice. RESULTS C57BL/6J mice developed hearing loss at 12M by ABR threshold shifting to (75±10), (78±26) and (81±14)dB SPL at frequencies of 8, 16 and 32 kHz; PCR showed that the relative expression of NKCC1 and Na-K-ATPase mRNA in the aged group decreased, which were 0.52±0.06 and 0.35±0.04 times higher than those in the young control group, the difference was statistically significant(t =7.466 and 16.11, all P＜0.05). WB showed that relative expression of NKCC1 and Na-K-ATPase protein level in the aged group decreased by 0.79±0.02 and 0.68±0.05 times as much as that of the young control group, the difference was statistically significant(t =8.857 and 6.771, P all＜0.05). After applied with Furosemide and Ouabain to suppress the two ion transporters, the ABR threshold increased to (50±17), (53±21), (55±17)dB SPL and (56±6), (70±17), (73±6)dB SPL at frequencies of 8, 16 and 32 kHz. CONCLUSION In vivo experiment of C57BL/6J suggested that NKCC1 and Na-K-ATPase might be related to age related hearing loss.
