Effect of silencing mitochondrial ribosomal protein L35 gene on growth of human esophageal cancer TE-1 cells
- VernacularTitle:沉默线粒体核糖体蛋白L35基因对人食管癌TE-1细胞生长的影响
- Author:
Aifu WANG
1
;
Qi ZHANG
;
Daoming ZHANG
;
Yuting XIU
;
Yaming DING
;
Linlin LIU
Author Information
1. 吉林大学第二医院放疗科
- Keywords:
mitochondrial ribosomal protein L35;
esophageal cancer TE-1 cells;
apoptosis
- From:
Journal of Jilin University(Medicine Edition)
2019;45(1):28-32,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of lentivirus-mediated silencing of mitochondrial ribosomal protein L35 (MRPL35) gene on the growth of human esophageal cancer TE-1cells, and to clarify its mechanism.Methods:Three kinds of human esophageal cancer cells, TE-1, ECA109and KYSE150, were selected.The relative expression levels of MRPL35mRNA in three kinds of cells by real-time quantitative PCR.The esophageal cancer TE-1cells were divided into shMRPL35group and shCtrl group, and the cells were infected with si-RNA lentivirus and si-RNA lentivirus;the esophageal cancer cell line stably silenting the MRPL35gene was established.Real-time quantitative PCR and Western blotting methods were used to detect the efficiency of MRPL35gene silencing.The cell growth curves in various groups were detected by CCK-8method, and the apoptotic rates were detected by flow cytometry after AnnexinⅤ-PE/7AAD double staining.Results:Three kinds of esophageal cancer cells expressed MRPL35gene, and the expression levels were not statistically significant between them (P＞0.05) .The results of real-time quantitative PCR and Western blotting methods showed that the mRNA and protein levels of MRPL35in the TE-1cells in shMRPL35group were significantly lower than those in shCtrl group (P＜0.05) .Compared with shCtrl group, the cell growth speed in shMRPL35group was decreased (P＜0.05) , and the apoptotic rate was significantly increased (P＜0.01) .Conclusion:Silencing MRPL35gene can inhibit the proliferation of esophageal cancer TE-1cells and plays a role through the apoptotic pathway.
