Transcriptome sequencing and analysis of bovine alveolar macrophages infected with BCG
- VernacularTitle:BCG感染牛肺泡巨噬细胞的转录组测序和分析
- Author:
Jinrui XU
1
;
Cuiping LYU
;
Yi YANG
;
Yanbing ZHOU
;
Jia LUO
;
Yujiong WANG
Author Information
1. 宁夏大学 西部特色生物资源保护与利用教育部重点实验室
- Keywords:
Bacillus Calmette-Guérin;
bovine alveolar macrophage;
transcriptome sequencing;
long non-coding RNA
- From:
Journal of Jilin University(Medicine Edition)
2019;45(1):12-16,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To reveal the differentially expressed genes and long non-coding RNAs (lncRNAs) by sequencing the transcriptome of bovine alveolar macrophages (BAM) infected with Bacillus Calmette-Guérin (BCG) and analyzing their bioinformations, and to provide theoretical foundation for the research on immune regulation mechanism of anti-infection of Mycobacteriumtuberculosisof macrophages.Methods:The BAM were collected by pulmonary lavage and centrifugation and cultured and divided into infected group and uninfected group.After infection for 12hin infected group, the expression profiles of mRNA and lncRNA in infected group and uninfected group were detected by RNA-Seq, and bioinformatics analysis was carried out.Results:compared with uninfected group, there were 119differentially expressed lncRNAs and 1111differentially expressed mRNA in infected group (P＜0.05) .Gene Ontology functional enrichment analysis showed that the most significant enrichment was immune response (GO:0006955, P＜0.05) , including 125genes, in which 63 were up-regulated and 62 were downregulated, and the expressions of proinflammatory factors interleukin-1 (IL-6) , interleukin-7 (IL-7) , and interleukin-23A (IL-23A) were up-regulated.Cis target gene prediction and KEGG pathway analysis showed that the differentially expressed lncRNAs were involved in the regulation of transforming growth factor-β (TGF-β) signaling pathway and ATP-binding cassette transporter (ABC transporter) signaling pathway (P＜0.05) .Conclusion:The host cells are stimulated to produce a strong immune response after the BAM are infected by BCG and results in the changes of lncRNA and mRNA expression profiles.
