A research for a newly discovered TMPRSS4-isoforms and its biological charcteristics in transfected colon cancer cells
10.19405/j.cnki.issn1000-1492.2019.02.005
- VernacularTitle:TMPRSS4亚型的新发现及其对转染结肠癌细胞的生物学特性研究
- Author:
Xuefeng ZHAO
1
;
Guangda XU
Author Information
1. 大连大学附属新华医院普通外科
- Keywords:
TMPRSS4-isoforms;
colon cancer cells;
biological charcteristics
- From:
Acta Universitatis Medicinalis Anhui
2019;54(2):187-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a newly discovered transmembrane protease serine 4 (TMPRSS4) isoforms and its molecular charcteristics in transfected colon cancer cells. Methods The named T4-1A and T4-1B of the TMPRSS4-isoforms were authenticated by the RT-PCR and Western blot,and then transfected to human colon cancer cells (DLD-1). Those stable transfected cells of migration and invasion were illustrated using wound healing assays and matrigel invasion assays. Results Successfully constructed T4-1A and T4-1B recombinant vectors,and obtained T4-1A and T4-1B transfected cell lines,and their T4-1A and T4-1B were highly expressed in stable DLD-1. Compared to cells transfected with empty vector of pcDNA6,the transfected DLD-1 with T4-1A enhanced migration and invasion were statistical significance (P < 0. 05). However,compared to pcDNA6 no significant1 difference was found for T4-1B. Moreover,the biological characteristics of T4-1A and TMPRSS4 were very similar. Conclusion The T4-1A and T4-1B is newly TMPRSS4-isoforms,and protease domain included to a T4-1A has further facilitated the migration and invasion of colon cancer cells,and further studies provide a theoretic base in the molecular biomedical characteristics of TMPRSS4.
