Value of polypeptide-based nanomagnetic circulating tumor cells detection for the differential diagnosis of pulmonary nodules

LI Kaidi; Liang Naixin; Liu Hongsheng; LI Li; Huang Cheng; Qin Yingzhi; Han Zhijun; Bing Zhongxing; Liu Lei; XU Yuan; XU Huihui; Yang Yanlian; Peng Jiaxi; Huo Li; LI Fang; HU Zhiyuan; LI Shanqing

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Value of polypeptide-based nanomagnetic circulating tumor cells detection for the differential diagnosis of pulmonary nodules

VernacularTitle:基于多肽的循环肿瘤细胞纳米磁珠检测对肺部结节的鉴别诊断价值
Author: LI Kaidi ^{1
,

2} ; LIANG Naixin ^{1
,

2} ; LIU Hongsheng ^{1
,

2} ; LI Li ^{1
,

2} ; HUANG Cheng ^{1
,

2} ; QIN Yingzhi ^{1
,

2} ; HAN Zhijun ^{1
,

2} ; BING Zhongxing ^{1
,

2} ; LIU Lei ^{1
,

2} ; XU Yuan ^{1
,

2} ; XU Huihui ³ ; YANG Yanlian ³ ; PENG Jiaxi ³ ; HUO Li ^{2
,

4} ; LI Fang ^{2
,

4} ; HU Zhiyuan ³ ; LI Shanqing ^{1
,

2}
Author Information

1. Department of Thoracic Surgery, Peking Union Medical College Hospital, CAMS &
2. PUMC, Beijing, 100730, P.R.China
3. National Center for Nanoscience and Technology, Beijing, 100190, P.R.China
4. Department of Nuclear Medicine, Peking Union Medical College Hospital, CAMS &
Publication Type:Journal Article
Keywords: Pulmonary nodules; differential diagnosis; diagnostic tests; liquid biopsy; circulating tumor cells
From: Chinese Journal of Clinical Thoracic and Cardiovascular Surgery 2018;25(7):560-566
CountryChina
Language:Chinese
Abstract: Objective To explore the efficacy of a novel detection technique of circulating tumor cells (CTCs) to identify benign and malignant lung nodules. Methods Nanomagnetic CTC detection based on polypeptide with epithelial cell adhesion molecule (EpCAM)-specific recognition was performed on enrolled patients with pulmonary nodules. There were 73 patients including 48 patients with malignant lesions as a malignant group and 25 patients with benign lesion as a benign group. There were 13 males and 35 females at age of 57.0±11.9 years in the malignant group and 11 males and 14 females at age of 53.1±13.2 years in the benign group. e calculated the differential diagnostic efficacy of CTC count, and conducted subgroup analysis according to the consolidation-tumor ratio, while compared with PET/CT on the efficacy. Results CTC count of the malignant group was significantly higher than that of the benign group (0.50/ml vs. 0.00/ml, P<0.05). Subgroup analysis according to consolidation tumor ratio (CTR) revealed that the difference was statistically significant in pure ground glass (pGGO) nodules 1.00/ml vs. 0.00/ml, P<0.05), but not in part-solid or pure solid nodules. For pGGO nodules, the area under the receiver operating characteristic (ROC) curve of CTC count was 0.833, which was significantly higher than that of maximum of standardized uptake value (SUVmax) (P<0.001). Its sensitivity and specificity was 80.0% and 83.3%, respectively. Conclusion The peptide-based nanomagnetic CTC detection system can differentiate malignant tumor and benign lesions in pulmonary nodules presented as pGGO. It is of great clinical potential as a noninvasive, nonradiating method to identify malignancies in pulmonary nodules.