The Long Noncoding RNA NEAT1 Targets miR-34a-5p and Drives Nasopharyngeal Carcinoma Progression via Wnt/β-Catenin Signaling
10.3349/ymj.2019.60.4.336
- Author:
Yuqing JI
1
;
Man WANG
;
Xueshen LI
;
Fusheng CUI
Author Information
1. Ear-Nose-Throat Department, Xingtai People's Hospital, Xingtai, China.
- Publication Type:Original Article
- Keywords:
Nasopharyngeal carcinoma;
lncRNA NEAT1;
miR-34a-5p;
Wnt/β-catenin signaling pathway
- MeSH:
Blotting, Western;
Cell Line;
Cell Movement;
Cell Proliferation;
Humans;
Immunoprecipitation;
In Vitro Techniques;
MicroRNAs;
Models, Animal;
Oncogenes;
Real-Time Polymerase Chain Reaction;
RNA;
RNA, Long Noncoding;
Sincalide
- From:Yonsei Medical Journal
2019;60(4):336-345
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) has been deemed an oncogene in many human cancers. However, the underlying mechanism of NEAT1 in nasopharyngeal carcinoma (NPC) progression remains largely unclear. MATERIALS AND METHODS: Quantitative real-time PCR assay was performed to assess the expression of NEAT1 and miR-34a-5p in NPC tissues and cells. Western blot analysis was used to observe cell epithelial to mesenchymal transition (EMT) and the activation of Wnt/β-catenin signaling in 5-8F cells. MiRNA directly interacting with NEAT1 were verified by dual-luciferase reporter assay and RNA immunoprecipitation. Cell proliferation ability was determined by CCK-8 assay, and cell migration and invasion capacities were assessed by transwell assays. An animal model was used to investigate the regulatory effect of NEAT1 on tumor growth in vivo. RESULTS: Our data revealed that NEAT1 is upregulated, while miR-34a-5p is downregulated in NPC tissues and cell lines. NEAT1 knockdown repressed tumor growth in vitro and in vivo. Additionally, we discovered that NEAT1 directly binds to miR-34a-5p and suppresses miR-34a-5p expression. Moreover, NEAT1 knockdown exerted suppression effects on cell proliferation, migration, invasion, and EMT by miR-34a-5p. NEAT1 knockdown blocked Wnt/β-catenin signaling via miR-34a-5p. CONCLUSION: Our study demonstrated that NEAT1 targets miR-34a-5p at least partly to drive NPC progression by regulating Wnt/β-catenin signaling, suggesting a potential therapeutic target for NPC.
