Determination of donepezil in human plasma using ultra performance liquid chromatography-tandem mass spectrometry
10.12793/tcp.2018.26.2.64
- Author:
Hyeon Cheol JEONG
1
;
Jeong Eun PARK
;
Ji Yeon HYUN
;
Min Kyu PARK
;
Dong Seong SHIN
;
Kwang Hee SHIN
Author Information
1. College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Korea. kshin@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Acetylcholinesterase inhibitor;
Donepezil;
Human plasma;
Method validation;
UPLC-MS/MS
- MeSH:
Acetic Acid;
Administration, Oral;
Ammonium Compounds;
Humans;
Liquid-Liquid Extraction;
Mass Spectrometry;
Methods;
Plasma
- From:Translational and Clinical Pharmacology
2018;26(2):64-72
- CountryRepublic of Korea
- Language:English
-
Abstract:
An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantification of donepezil in human plasma. Donepezil and donepezil-D4 were extracted from human plasma by liquid-liquid extraction using a mixture of hexane and ethyl acetate (70:30 v/v). The extracted samples were analyzed using a Thermo Hypersil Gold C18 column with 5% acetic acid in 20 mM ammonium acetate buffer (pH 3.3) and 100% acetonitrile as a mobile phase with the 60:40 (v:v) isocratic method, at a flow rate of 0.3 mL/min. The injection volume was 3 µL, and the total run time was 3 min. Inter- and intra-batch accuracies ranged from 98.0% to 110.0%, and the precision was below 8%. The developed method was successfully applied to the quantification of donepezil in human plasma. The mean (standard deviation) maximum concentration and the median (range) time to maximum concentration were 8.6 (2.0) ng/mL and 2.0 h (1.0~5.0 h), respectively, in healthy Koreans after oral administration of 5 mg donepezil.
