In Vivo Safety and Regeneration of Long-Term Transported Amniotic Fluid Stem Cells for Renal Regeneration
10.1007/s13770-018-0162-6
- Author:
Na hee YU
1
;
So Young CHUN
;
Yun Sok HA
;
Hyun Tae KIM
;
Eugene LIH
;
Dae Hwan KIM
;
Jeongshik KIM
;
Jae Wook CHUNG
;
Phil Hyun SONG
;
Eun Sang YOO
;
Sung Kwang CHUNG
;
Dong Keun HAN
;
Bum Soo KIM
;
Tae Gyun KWON
Author Information
1. BioMedical Research Institute, Kyungpook National University Hospital, 130 Dongdeok-ro, Jung-gu, Daegu 41944, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Amniotic fluid stem cell;
Long-term cell transportation;
Tissue regeneration
- MeSH:
Amniotic Fluid;
Blood Urea Nitrogen;
Cell- and Tissue-Based Therapy;
Creatinine;
Cytokines;
Female;
In Vitro Techniques;
Kidney;
Liver;
Lung;
Polyglactin 910;
Regeneration;
Spleen;
Stem Cells
- From:
Tissue Engineering and Regenerative Medicine
2019;16(1):81-92
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Despite major progress in stem cell therapy, our knowledge of the characteristics and tissue regeneration potency of long-term transported cells is insufficient. In a previous in vitro study, we established the optimal cell transport conditions for amniotic fluid stem cells (AFSCs). In the present study, the target tissue regeneration of long-term transported cells was validated in vivo. METHODS: For renal regeneration, transported AFSCs were seeded on a poly(lactide-co-glycolide) scaffold and implanted in a partially resected kidney. The target tissue regeneration of the transported cells was compared with that of freshly harvested cells in terms of morphological reconstruction, histological microstructure reformation, immune cell infiltration, presence of induced cells, migration into remote organs, expression of inflammation/fibrosis/renal differentiation-related factors, and functional recovery. RESULTS: The kidney implanted with transported cells showed recovery of total kidney volume, regeneration of glomerular/renal tubules, low CD4/CD8 infiltration, and no occurrence of cancer during 40 weeks of observation. The AFSCs gradually disappeared and did not migrate into the liver, lung, or spleen. We observed low expression levels of proinflammatory cytokines and fibrotic factors; enhanced expression of the genes Wnt4, Pax2, Wt1, and Emx2; and significantly reduced blood urea nitrogen and creatinine values. There were no statistical differences between the performance of freshly harvested cells and that of the transported cells. CONCLUSION: This study demonstrates that long-term transported cells under optimized conditions can be used for cell therapy without adverse effects on stem cell characteristics, in vivo safety, and tissue regeneration potency.
