Human Embryonic Stem Cells-Derived Mesenchymal Stem Cells Reduce the Symptom of Psoriasis in Imiquimod-Induced Skin Model
10.1007/s13770-018-0165-3
- Author:
Chang Hyun KIM
1
;
Chi Yeon LIM
;
Ju Hee LEE
;
Keun Cheon KIM
;
Ji Yeon AHN
;
Eun Ju LEE
Author Information
1. College of Medicine, Dongguk University, 32 Donggukro, Ilsandonggu, Goyang, Gyeonggi 10326, Republic of Korea.
- Publication Type:Original Article
- Keywords:
hE-MSCs;
Skin psoriasis;
Immune modulation;
Psoriasis
- MeSH:
Animals;
Cytokines;
Embryonic Stem Cells;
Humans;
Lymphocytes;
Mesenchymal Stromal Cells;
Mice;
Psoriasis;
Skin Diseases;
Skin;
Splenomegaly;
Tissue Donors
- From:
Tissue Engineering and Regenerative Medicine
2019;16(1):93-102
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Mesenchymal stem cells (MSCs) can be used for a wide range of therapeutic applications because of not only their differentiation potential but also their ability to secrete bioactive factors. Recently, several studies have suggested the use of human embryonic stem cell-derived MSCs (hE-MSCs) as an alternative for regenerative cellular therapy due to mass production of MSCs from a single donor. METHODS: We generated hE-MSCs from embryonic stem cell lines, SNUhES3, and analyzed immune properties of these cells. Also, we evaluated the in-vivo therapeutic potential of hE-MSCs in immune-mediated inflammatory skin disease. RESULTS: The cell showed the suppression of immunity associated with allogenic peripheral blood mononuclear cells in mixed lymphocyte response assay. We also detected that cytokines and growth factor related to the immune response were secreted from these cells. To assessed the in-vivo therapeutic potential of hE-MSCs in immune-mediated inflammatory skin disease, we used imiquimod (IMQ)-induced skin psoriasis mouse model. The score of clinical skin was significantly reduced in the hE-MSCs treated group compared with control IMQ group. In histological analysis, the IMQ-induced epidermal thickness was significantly decreased by hE-MSCs treatment. It was correlated with splenomegaly induced by IMQ which was also improved in the hE-MSCs. Moreover, IMQ-induced inflammatory cytokines; Th1 cytokines (TNF-α, IFN-α, IFN-γ,and IL-27) and Th17 cytokines (IL-17A and IL-23), in the serum and skin showed marked inhibition by hEMSCs. CONCLUSION: These results suggested that hE-MSCs have a potency of immune modulation in psoriasis, which might be the key factor for the improved psoriasis.
