Oral Immunization of FMDV Vaccine Using pH-Sensitive and Mucoadhesive Thiolated Cellulose Acetate Phthalate Microparticles
10.1007/s13770-017-0082-x
- Author:
Ho Bin LEE
1
;
So Yeon YOON
;
Bijay SINGH
;
Seo Ho OH
;
Lianhua CUI
;
Changguo YAN
;
Sang Kee KANG
;
Yun Jaie CHOI
;
Chong Su CHO
Author Information
1. Department of Agricultural Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea. cyjcow@snu.ac.kr, chocs@snu.ac.kr
- Publication Type:Original Article
- Keywords:
pH-senstive;
Mucoadhesive;
Foot-and-mouth disease;
Subunit vaccine;
Oral immunization
- MeSH:
Animals;
Cellulose;
Foot-and-Mouth Disease;
Gastrointestinal Tract;
Hydrogen-Ion Concentration;
Immunization;
Immunoglobulin A;
Intestine, Small;
Livestock;
Peyer's Patches;
Polymers;
Staphylococcal Protein A;
Stomach
- From:
Tissue Engineering and Regenerative Medicine
2018;15(1):1-11
- CountryRepublic of Korea
- Language:English
-
Abstract:
Several barriers such as gastric pH, enzymatic degradation and rapid transit should be overcome to orally deliver antigens for taking up by epithelial microfold cells in Peyer's patches of small intestine. To solve the above mentioned problems, we designed pH-sensitive and mucoadhesive polymeric microparticles (MPs) prepared by double emulsion technique using cellulose acetate phthalate (CAP) to enhance immune response of foot-and-mouth disease (FMD) virus (FMDV) subunit vaccine. Thiolation of CAP improved mucoadhesive property of CAP to prolong the MPs transit time through the gastrointestinal tract. Thiolated CAP (T-CAP) also slowed down antigen release in acidic pH of stomach but released more antigens in neutral pH of small intestine due to the pH-sensitivity of the T-CAP. Oral immunization of a chimerical multi-epitope recombinant protein as the FMD subunit vaccine via T-CAP MPs effectively delivered the vaccine to Peyer's patches eliciting mucosal IgA response. It will make a step forward into a promising oral subunit vaccine development in livestock industry.
