Adipose Stem Cells with Conditioned Media for Treatment of Acne Vulgaris Scar
10.1007/s13770-017-0105-7
- Author:
Xing SHAN
1
;
Jong Hyeon CHOI
;
Ki Joo KIM
;
Yoon Jae LEE
;
Yeon Hee RYU
;
Su Jin LEE
;
Suk Ho MOON
;
Jong Won RHIE
Author Information
1. Department of Plastic and Reconstructive Surgery, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea. rhie@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Acne vulgaris scar;
Adipose stem cell;
Acne scar model;
Conditioned media;
Keratin 16
- MeSH:
Acne Vulgaris;
Adipose Tissue;
Adult;
Blotting, Western;
Cicatrix;
Culture Media, Conditioned;
Ear;
Eosine Yellowish-(YS);
Hematoxylin;
Humans;
Immunohistochemistry;
Inflammation;
Injections, Subcutaneous;
Keratin-16;
Male;
Matrix Metalloproteinase 2;
Methods;
Models, Animal;
Necrosis;
New Zealand;
Rabbits;
Re-Epithelialization;
RNA;
Scapula;
Stem Cells
- From:
Tissue Engineering and Regenerative Medicine
2018;15(1):49-61
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was to investigate the effect of subcutaneous injection of the adipose stem cells (ASCs) with conditioned media (CM) in the treatment of acne vulgaris scar. We used Adult male New Zealand white rabbit ears as an animal model and induced acne formation by Kignman method. Adipose tissue was isolated and harvested from the scapula of rabbits, and ASCs were cultured and expanded until passage 1. There have four groups in our experiment, include phosphate buffered saline (PBS), ASCs with PBS (ASC + PBS), CM, and ASCs with CM (ASC + CM) group. This solution of 0.6 ml injected to subcutaneous in each group. ASC + PBS and ASC + CM groups were containing ASCs of 5.0 × 106 cells/ml. We analyzed the treatment of 4 groups to scar tissue after 2 and 4 weeks by hematoxylin and eosin stain, immunohistochemistry, and RNA expression level of tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α), and matrix metalloproteinase-2 (MMP-2). Also, the expression of keratin 16 (K16) was detected by western blot analysis. H&E stain showed that infiltration of inflammation cells was significantly reduced at 2 and 4 weeks, as well as re-epithelialization was improved in the ASC + CM group. The ASC + CM gourp was reduced both expression levels of TNF-α, IL-1α, and MMP-2 and K16 protein level. In conclusion, the ASCs with CM has a significant curative effect on acne vulgaris scar, more to the point, the CM has a key role on treatment. It could be applied to a therapeutic approach to regenerate to treat acne vulgaris scar.
