A Novel Niosomal Combination of Selenium Coupled with Glucantime against Leishmania tropica
- Author:
Mahshid MOSTAFAVI
1
;
Payam KHAZAELI
;
Iraj SHARIFI
;
Saeedeh FARAJZADEH
;
Hamid SHARIFI
;
Alireza KEYHANI
;
Maryam Hakimi PARIZI
;
Sina KAKOOEI
Author Information
- Publication Type:Original Article
- Keywords: Leishmania tropica; selenium; glucantime; niosomes; anti-leishmanial effect; cytokine
- MeSH: Gene Expression; In Vitro Techniques; Interleukin-12; Interleukins; Leishmania tropica; Leishmania; Leishmaniasis; Liposomes; Selenium; Transcriptome
- From:The Korean Journal of Parasitology 2019;57(1):1-8
- CountryRepublic of Korea
- Language:English
- Abstract: There is no effective treatment modality available against different forms of leishmaniasis. Therefore, the aim of this study was to improve the penetration and efficacy of selenium and glucantime coupled with niosomes and compared them with their simple forms alone on in vitro susceptibility assays. In this study, the niosomal formulations of selenium and in combination with glucantime were prepared. The size and morphology of the niosomal formulations were characterized and the effectivity of the new formulation was also evaluated using in vitro MTT assay, intra-macrophage model, and gene expression profile. From the results obtained, no cytotoxicity effect was observed for niosomal and simple forms of drugs, as alone or in combination. Niosomal formulations of the drugs significantly showed more inhibitory effects (P≤0.001) than the simple drugs when the selectivity index was considered. The gene expression levels of Interleukin (IL-10) significantly decreased, while the level of IL-12 and metacaspase significantly increased (P≤0.001). The results of the present study showed that selenium plus glucantime niosome possess a potent anti-leishmanial effect and enhanced their lethal activity as evidenced by the in vitro experiments.
